Vitamin D receptor expression in human muscle tissue decreases with age
Vitamin D receptor expression in human muscle tissue decreases with age
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DOI：
10.1359/jbmr.2004.19.2.265
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发表时间：
2004-02-01
影响因子：
6.2
通讯作者：
Dick, W
中科院分区：
文献类型：
--
作者：
Bischoff-Ferrari, HA;Borchers, M;Dick, W
简介：我们研究了这些因素及其与1，25-二羟维生素D受体（VDR）在新鲜切除的人体肌肉组织中表达的关系。材料和方法：我们研究了20例接受全髋关节置换术的女性患者手术时取的臀中肌活检标本（平均年龄，71.6 ± 14.5岁; 72% > 65岁）和手术时从12名接受脊柱手术的女性患者中采集的横棘肌活检标本（平均年龄：55.2 ± 19.6岁; 28% > 65岁）。使用VDR的单克隆大鼠抗体（克隆编号9A 7）对VDR进行免疫组织学染色，获得标本。通过对每个标本和每个人计数500个细胞核来评估VDR定量表达（VDR阳性细胞核的数量）。血清25-羟维生素D和1，25-二羟维生素D的浓度进行了评估，在入院当天的surging.Results：所有肌肉活检标本染色阳性VDR。在单变量分析中，年龄增加与VDR表达降低相关（r = 0.5：p = 0.004），而VDR表达与25-羟基维生素D或1，25-二羟维生素D水平之间无显著相关性。VDR表达在髋关节和脊柱手术患者之间没有差异。在多变量分析中，控制活检部位（臀中肌或横棘肌）和25-羟基维生素D水平后，年龄较大是VDR表达降低的重要预测因素（线性回归分析：β-估计值= -2.56; p = 0.047）。年龄较大与VDR表达降低显著相关，与活检部位和血清25-羟维生素D水平无关。
Introduction: We investigated these factors and their relation to 1,25-dihydroxyvitamin D receptor (VDR) expression in freshly removed human muscle tissue.Materials and Methods: We investigated biopsy specimens of the gluteus medius taken at surgery from 20 female patients undergoing total hip arthroplasty (mean age, 71.6 +/- 14.5; 72% > 65 years) and biopsy specimens of the transversospinalis muscle taken at surgery from 12 female patients with spinal operations (mean age, 55.2 +/- 19.6; 28% > 65 years). The specimens were obtained by immunohistological staining of the VDR using a monoclonal rat antibody to the VDR (Clone no. 9A7). Quantitative VDR expression (number of VDR positive nuclei) was assessed by counting 500 nuclei per specimen and person. Serum concentrations of 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D were assessed at day of admission to surgery.Results: All muscle biopsy specimens stained positive for VDR. In the univariate analyses, increased age was associated with decreased VDR expression (r = 0.5: p = 0.004), whereas there were no significant correlations between VDR expression and 25-hydroxyvitamin D or 1,25-dihydroxyvitamin D levels. VDR expression did not differ between patients with hip and spinal surgery. In the multivariate analysis, older age was a significant predictor of decreased VDR expression after controlling biopsy location (gluteus medius or the transversospinalis muscle), and 25-hydroxyvitamin D levels (linear regression analysis: beta-estimate = -2.56; p = 0.047).Conclusions: Intranuclear immunostaining of the VDR was present in muscle biopsy specimens of all orthopedic patients. Older age was significantly associated with decreased VDR expression, independent of biopsy location and serum 25-hydroxyvitamin D levels.