Large area laser scanning optical resolution photoacoustic microscopy using a fibre optic sensor.
Large area laser scanning optical resolution photoacoustic microscopy using a fibre optic sensor.
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DOI：
10.1364/boe.9.000650
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发表时间：
2018-02-01
影响因子：
3.4
通讯作者：
Beard PC
中科院分区：
文献类型：
--
作者：
Allen TJ;Ogunlade O;Zhang E;Beard PC
介绍了一种基于固定式光纤传感器的激光扫描光声显微镜（LS OR-PAM）系统。该传感器包括形成于圆形单模光纤尖端上的光学谐振干涉聚合物腔。它提供低噪声等效压力（NEP = 68.7 Pa在20 MHz测量带宽），扩展到80 MHz的宽带宽和近全向响应。后者是一个显著的优势，因为它可以在不需要平移机械扫描的情况下对大面积（>1cm2）进行成像，从而提供了快速图像采集的潜力。该系统的横向分辨率为8µm，轴向分辨率为21µm，视野范围为10mm × 10mm。为了演示该系统，获得了小鼠耳朵微血管的体内3D结构图像，显示了覆盖在大血管上的单个毛细血管以及显示血氧饱和度的功能图像。
A laser scanning optical resolution photoacoustic microscopy (LS OR-PAM) system based on a stationary fibre optic sensor is described. The sensor comprises an optically resonant interferometric polymer cavity formed on the tip of a rounded single mode optical fibre. It provides low noise equivalent pressure (NEP = 68.7 Pa over a 20 MHz measurement bandwidth), a broad bandwidth that extends to 80 MHz and a near omnidirectional response. The latter is a significant advantage, as it allows large areas (>1cm2) to be imaged without the need for translational mechanical scanning offering the potential for fast image acquisition. The system provides a lateral resolution of 8 µm, an axial resolution of 21 µm, and a field of view up to 10 mm × 10 mm. To demonstrate the system, in vivo 3D structural images of the microvasculature of a mouse ear were obtained, showing single capillaries overlaying larger vessels as well as functional images revealing blood oxygen saturation.