A comprehensive study into the impact of a chitosan mouthwash upon oral microorganism's biofilm formation in vitro
A comprehensive study into the impact of a chitosan mouthwash upon oral microorganism's biofilm formation in vitro
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DOI：
10.1016/j.carbpol.2013.09.041
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发表时间：
2014-01-30
影响因子：
11.2
通讯作者：
Pintado, M. M.
中科院分区：
文献类型：
--
作者：
Costa, E. M.;Silva, S.;Pintado, M. M.
现代牙科强调控制牙菌斑对改善口腔健康的重要性。为此，口腔护理制剂的开发已经面向并入在口腔健康维护中可能起关键作用的抗牙斑剂。近年来，对抗牙斑剂的研究发现了具有显著影响生物膜形成能力的化合物。在这些化合物中，壳聚糖是一种多糖，其表现出很强的干扰变形链球菌生物膜形成的能力。因此，这项工作的目的是将壳聚糖纳入漱口水基质，并评估其对口腔微生物生物膜形成的影响。通过研究漱口水对微生物粘附、生物膜形成和成熟生物膜的影响进行该评估。此外，壳聚糖漱口水的作用与两种市售漱口水进行了比较。这里获得的结果表明，只有含壳聚糖的漱口水能够干扰所有微生物的粘附、生物膜形成和成熟的生物膜，同时显示出比所测定的两种商业漱口水显著优越的上级活性。因此，壳聚糖漱口水显示出作为传统漱口水的天然和有效替代品的巨大潜力。(C)2013爱思唯尔有限公司保留所有权利。
Modern dentistry emphasizes the importance of dental plaque control to improve oral health. To that end the development of oral care formulations has been geared toward the incorporation of antiplaque agents that may play a crucial role in oral health maintenance. In later years the research into antiplaque agents has led to the discovery of compounds with significant capability to affect biofilm formation. Among these compounds was chitosan, a polysaccharide which showed great ability to interfere with Streptococcus mutans biofilm formation. As such the aim of this work was to incorporate chitosan into a mouthwash matrix and assess its effect upon biofilm formation of oral microorganisms. This assessment was performed via study of the impact the mouthwash upon microbial adherence, biofilm formation and mature biofilms. Additionally, the action of the chitosan mouthwash was compared with two commercially available mouthwashes. The results here obtained show that only the chitosan containing mouthwash was capable of interfering with all microorganisms' adherence, biofilm formation and mature biofilms while at the same time showing vastly superior activity than both commercial mouthwashes assayed. As such a chitosan mouthwash shows great potential as a natural and efficient alternative to traditional mouthwashes. (C) 2013 Elsevier Ltd. All rights reserved.