Ethylene-induced stomatal closure in Arabidopsis occurs via AtrbohF-mediated hydrogen peroxide synthesis
Ethylene-induced stomatal closure in Arabidopsis occurs via AtrbohF-mediated hydrogen peroxide synthesis
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DOI：
10.1111/j.1365-313x.2006.02842.x
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发表时间：
2006-09-01
期刊：
影响因子：
7.2
通讯作者：
Neill, Steven J.
中科院分区：
文献类型：
--
作者：
Desikan, Radhika;Last, Kathryn;Neill, Steven J.
乙烯是一种调节植物生长发育许多方面的激素。尽管众所周知乙烯和应激信号之间存在关联，但其对气孔运动的影响在很大程度上尚未被探索。在这里，提供的遗传和生理数据表明，乙烯进入拟南芥保护细胞信号网络，并证明了乙烯和过氧化氢（H2O2）之间的功能联系。在野生型叶片中，乙烯诱导气孔关闭依赖于保护细胞产生H2O2，由烟酰胺腺嘌呤二核苷酸磷酸氢（NADPH）氧化酶AtrbohF产生。乙烯诱导的关闭被乙烯拮抗剂1-MCP和银抑制。乙烯受体突变体etr1-1和etr1-3在气孔关闭和H2O2产生方面对乙烯不敏感。乙烯信号突变体ein2-1和arr2的气孔在乙烯或H2O2胁迫下都不会关闭，但在乙烯胁迫下会产生H2O2。因此，这些数据表明，保护细胞中的乙烯和H2O2信号是由ETR1通过EIN2和arr2依赖途径介导的，并确定了AtrbohF是气孔对乙烯响应的关键介质。
Ethylene is a plant hormone that regulates many aspects of growth and development. Despite the well-known association between ethylene and stress signalling, its effects on stomatal movements are largely unexplored. Here, genetic and physiological data are provided that position ethylene into the Arabidopsis guard cell signalling network, and demonstrate a functional link between ethylene and hydrogen peroxide (H2O2). In wild-type leaves, ethylene induces stomatal closure that is dependent on H2O2 production in guard cells, generated by the nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase AtrbohF. Ethylene-induced closure is inhibited by the ethylene antagonists 1-MCP and silver. The ethylene receptor mutants etr1-1 and etr1-3 are insensitive to ethylene in terms of stomatal closure and H2O2 production. Stomata of the ethylene signalling ein2-1 and arr2 mutants do not close in response to either ethylene or H2O2 but do generate H2O2 following ethylene challenge. Thus, the data indicate that ethylene and H2O2 signalling in guard cells are mediated by ETR1 via EIN2 and ARR2-dependent pathway(s), and identify AtrbohF as a key mediator of stomatal responses to ethylene.