Surface Investigation on Biomimetic Materials to Control Cell Adhesion: The Case of RGD Conjugation on PCL
Surface Investigation on Biomimetic Materials to Control Cell Adhesion: The Case of RGD Conjugation on PCL
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DOI：
10.1021/la100207q
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发表时间：
2010-06-15
期刊：
影响因子：
3.9
通讯作者：
Netti, Paolo A.
中科院分区：
文献类型：
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作者：
Causa, Filippo;Battista, Edmond;Netti, Paolo A.
固定在聚合物表面上的生物活性配体的细胞识别强烈依赖于在细胞/材料界面处的配体呈递。虽然小肽序列，如精氨酸甘氨酸天冬氨酸（RGD）被广泛用于获得仿生界面，固定化后的表面特性，以及这种配体的细胞受体的介绍值得更详细的调查。在这里，我们将基于RGD的序列固定在聚（ε-己内酯）（PC 1..）上，在聚合物氨解之后，在生物医学应用中广泛使用的聚合物材料。通过表面分析（FTIR-ATR、接触角测量、表面自由能测定）和分光光度测定来监测表面特性沿着官能化的功效，所述分光光度测定特别适用于界面处的官能团和/或肽的分析定量。特别注意的是评价的数量，形态，和渗透深度的固定化官能团和/或肽接枝在聚合物基板上。特别是，一个典型的形态，在肽分布的表面上证明了从聚合物微晶提出，而一个显着的渗透深度的接枝分子被揭示。NIH 3 T3成纤维细胞粘附研究证实了配体的正确呈递，肽缀合后细胞粘附增强。这些工作提出了一种表面表征的形态学和分析方法，以研究固定在聚合物基底上的配体的表面处理和分布。
The cell recognition of bioactive ligands immobilized on polymeric surfaces is strongly dependent on ligand presentation at the cell/material interface. While small peptide sequences such as Arg Gly Asp (RGD) are being widely used to obtain biomimetic interfaces, surface characteristics after immobilization as well as presentation of such ligands to cell receptors deserve more detailed investigation. Here, we immobilized an RGD-based sequence on poly(e-caprolactone) (PC1..), a largely widespread polymeric material used in biomedical applications, after polymer aminolysis. The surface characteristics along with the efficacy of the functionalization was monitored by surface analysis (FTIR-ATR, contact angle measurements, surface free energy determination) and spectrophotometric assays specially adapted for the analytical quantification of functional groups and/or peptides at the interface. Particular attention was paid to the evaluation of a number, morphology, and penetration depth of immobilized functional groups and/or peptides engrafted on polymeric substrates. In particular, a typical morphology in peptide distribution was evidenced on the surface raised from polymer crystallites, while a significant penetration depth of the engrafted molecules was revealed. NIH3T3 fibroblast adhesion studies verified the correct presentation of the ligand with enhanced cell attachment after peptide conjugation. Such work proposes a morphological and analytical approach in surface characterization to study the surface treatment and the distribution of ligands immobilized on polymeric substrates.