SEMLIKI FOREST VIRUS-INDUCED POLYKARYOCYTE FORMATION IS AN ATP-DEPENDENT EVENT
SEMLIKI FOREST VIRUS-INDUCED POLYKARYOCYTE FORMATION IS AN ATP-DEPENDENT EVENT
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DOI：
10.1007/bf01311338
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发表时间：
1987-01-01
影响因子：
2.7
通讯作者：
KOBLET, H
中科院分区：
文献类型：
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作者：
KEMPF, C;KOHLER, U;KOBLET, H
用Semkiki森林病毒（SFV）感染白纹伊蚊细胞导致在低于pH 6.2下形成多核细胞。这种合胞体的形成伴随着细胞ATP水平的降低。氧化磷酸化抑制剂的添加导致在pH 6下感染细胞中ATP的快速、完全耗尽，并导致多核细胞形成的抑制。然而，当细胞在抑制剂存在下暴露于pH 6仅几分钟，然后保持在pH 7.2时，ATP水平部分恢复到足以形成合胞体的值。用2-脱氧葡萄糖诱导ATP耗竭后也得到类似的结果。因此，可以得出结论，SFV诱导的合胞体形成是ATP依赖性事件。
Infection of Aedes albopictus cells with Semkiki Forest virus (SFV) leads to polykaryocyte formation below pH 6.2. This syncytim formation is accompanied by a decrease of the cellular ATP level. Addition of inhibitors of oxidative phosphorylation leads to a rapid, total depletion of ATP in infected cells at pH 6 and results in an inhibition of polykaryocyte formation. However, when cells were exposed for only a few minutes to pH 6 in the presence of the inhibitors and then kept at pH 7.2, the ATP level partially recovered to values sufficient for syncytium formation. Similar results were obtained after ATP depletion induced by 2-deoxyglucose. Thus, it can be concluded that SFV-induced syncytium formation is an ATP-dependent event.