Essential roles of ameloblastin in maintaining ameloblast differentiation and enamel formation
Essential roles of ameloblastin in maintaining ameloblast differentiation and enamel formation
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DOI：
10.1159/000091380
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发表时间：
2005-01-01
影响因子：
2.7
通讯作者：
Yamada, Y
中科院分区：
文献类型：
--
作者：
Fukumoto, S;Yamada, A;Yamada, Y
在牙齿发育过程中，牙上皮细胞与细胞外基质成分，如基底膜和釉质基质相互作用。成釉蛋白是一种釉基质蛋白，在维持成釉细胞分化状态中起着至关重要的作用，是釉质形成所必需的。成釉细胞蛋白基因敲除小鼠出现严重的釉质发育不全。在突变小鼠中，牙上皮细胞开始分化成成釉细胞，但成釉细胞很快失去细胞极性，增殖，并形成多个细胞层，表明成釉细胞表型的某些方面。此外，釉原蛋白的表达，釉基质的另一个组成部分，特别是在突变成釉细胞减少。超过20%的Amelobastin缺失小鼠发生牙源性肿瘤。我们还发现，重组成釉蛋白特异性结合成釉细胞和抑制牙上皮细胞的增殖。这些结果表明，成釉蛋白是维持成釉细胞分化状态的重要调节因子。版权所有（c）2005 S. Karger AG，巴塞尔。
During tooth development, dental epithelial cells interact with extracellular matrix components, such as the basement membrane and enamel matrix. Ameloblastin, an enamel matrix protein, plays a crucial role in maintaining the ameloblast differentiation state and is essential for enamel formation. Ameloblastin-null mice developed severe enamel hypoplasia. In mutant mice, dental epithelial cells started to differentiate into ameloblasts, but ameloblasts soon lost cell polarity, proliferated, and formed multiple cell layers, indicative of some aspects of preameloblast phenotypes. In addition, the expression of amelogenin, another component of the enamel matrix, was specifically reduced in mutant anneloblasts. More than 20% of amelobastin-null mice developed odontogenic tumors. We also found that recombinant ameloblastin specifically bound to anneloblasts and inhibited proliferation of dental epithelial cells. These results suggest that ameloblastin is an important regulator to maintain the differentiation state of anneloblasts. Copyright (c) 2005 S. Karger AG, Basel.