Regeneration of a germinal layer in the adult mammalian brain
Regeneration of a germinal layer in the adult mammalian brain
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DOI：
10.1073/pnas.96.20.11619
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发表时间：
1999-09-28
影响因子：
11.1
通讯作者：
Alvarez-Buylla, A
中科院分区：
文献类型：
--
作者：
Doetsch, F;García-Verdugo, JM;Alvarez-Buylla, A
神经元前体存在于成年哺乳动物的脑室下区（SVZ）。该区域由星形胶质细胞包裹的迁移成神经细胞链网络组成，并由未成熟前体（C型细胞）簇并置。在这里，我们表明，在用胞嘧啶-β-D-阿拉伯呋喃糖苷抗有丝分裂治疗后，神经母细胞和C型细胞被消除，但一些星形胶质细胞仍然存在。值得注意的是，SVZ网络迅速再生。胞嘧啶-β-D-阿拉伯呋喃糖苷处理后不久，星形胶质细胞分裂。两天后，C型细胞重新出现，随后在4.5天由迁移的神经母细胞。到10天，SVZ网络完全再生，迁移成神经细胞链的方向和组织与正常小鼠相似。这种再生揭示了一个意想不到的可塑性，在成人中枢神经系统，并应提供一个模型系统，以研究早期阶段的神经元：在成人大脑的发生。
Neuronal precursors reside in the subventricular zone (SVZ) of adult mammals. This region is composed of a network of chains of migrating neuroblasts ensheathed by astrocytes and juxtaposed by clusters of immature precursors (type C cells). Here we show that after antimitotic treatment with cytosine-beta-D-arabinofuranoside, neuroblasts and type C cells are eliminated but some astrocytes remain. Remarkably, the SVZ network rapidly regenerates. Soon after cytosine-beta-D-arabinofuranoside treatment astrocytes divide. Two days later, type C cells reappear, followed at 4.5 days by migrating neuroblasts. By 10 days the SVZ network is fully regenerated, and the orientation and organization of chains of migrating neuroblasts resemble that of normal mice. This regeneration reveals an unexpected plasticity in the adult central nervous system and should provide a model system to study the early stages of neuro: genesis in the adult brain.