Fibroblast-collagen interactions in the formation of the secondary stroma of the chick cornea.
Fibroblast-collagen interactions in the formation of the secondary stroma of the chick cornea.
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DOI：
10.1083/jcb.74.3.816
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发表时间：
1977-09
期刊：
影响因子：
--
通讯作者：
Higginson K
中科院分区：
文献类型：
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作者：
Bard JB;Higginson K
成纤维细胞侵入6日龄鸡胚眼的原代角膜基质。在随后的8天内，通过显微镜检查基质，研究了这些细胞构建次级基质的方式。将眼睛包埋在低粘度硝酸纤维素中，切下角膜的30微米切向切片并用azan染色（得到蓝色胶原和红细胞）。这些切片足够厚，以包括足够的细胞和胶原蛋白，用于在Nomarski光学系统下可见的基质组织。浸润后3天，成纤维细胞沿间质后部区域的沿着胶原束延伸;令人惊讶的是，上皮附近的成纤维细胞更圆。胶原蛋白本身组织成正交束而不是片。测量结果表明，后束的大小随着时间的推移而增加，而前间质的直径与初级间质相似。这些观察结果证实，上皮细胞继续沉积存款初级基质至少到第14天。此外，他们还表明成纤维细胞在现存的基质上沉积存款胶原纤维，并且纤维束离上皮越远，因此从它第一次铺设以来的时间越长，它可能越宽。对基质中透明质酸水平的结果和现有数据的分析表明，鲍曼氏膜，即前基质中保持未被细胞定殖的区域，至少在此期间是放置但尚未肿胀的初级基质。
Fibroblasts invade the primary corneal stroma of the 6-day-old chick embryo eye. The way in which these cells build the secondary stroma has been studied by microscope examination of the stroma during the subsequent 8 Days. Eyes were embedded in low viscosity nitrocellulose, and 30-micrometer tangential sections of cornea were cut and stained with azan (giving blue collagen and red cells). These sections were sufficiently thick to include enough cells and collagen for stromal organization to be visible under Nomarski optics. Three days after invasion, the fibroblasts extend along collagen bundles in the posterior region of the stroma; surprisingly, fibroblasts near the epithelium are more rounded. The collagen itself is organized in orthogonal bundles rather than in sheets. Measurements show that posterior bundles increase in size with time while anterior stroma si similar in diameter to primary stroma. These observations confirm that the epithelium continues to deposit primary stroma up to at least the 14th day. They show, moreover, that fibroblasts deposit collagen fibrils on extant stroma and that the farther a bundle is from the epithelium, and hence the longer the period since it was first laid down, the wider it is likely to be. Analysis of the results and existing data on hyaluronic acid levels in the stroma suggests that Bowman's membrane, the region of anterior stroma that remains uncolonized by cells, is, during this period at least, primary stroma laid down but as yet unswollen.