Dye-enhanced multimodal confocal microscopy for noninvasive detection of skin cancers in mouse models.
Dye-enhanced multimodal confocal microscopy for noninvasive detection of skin cancers in mouse models.
复制标题
染料增强多模态共聚焦显微镜用于小鼠模型皮肤癌的无创检测。
DOI：
10.1117/1.3394301
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发表时间：
2010
影响因子：
3.5
通讯作者：
Yaroslavsky,AnnaN
中科院分区：
文献类型：
--
作者：
Park,Jesung;Mroz,Pawel;Hamblin,MichaelR;Yaroslavsky,AnnaN
皮肤癌是人类癌症中最常见的形式。其早期诊断和及时治疗是至关重要的皮肤科和外科肿瘤。在这项研究中，我们评估使用反射和荧光共聚焦显微镜检测皮肤癌在anin-vivotrial与B16 F10黑色素瘤和SCCVII鳞状细胞癌小鼠。对于实验，将小鼠麻醉，然后用亚甲蓝水溶液浸润肿瘤并成像。反射率图像的获取。荧光在690和690之间的范围内激发并记录。成像后，处死小鼠。切除肿瘤并进行苏木精和伊红组织病理学处理，将其与光学图像进行比较。研究结果表明，在体反射图像提供了有价值的信息血管化的肿瘤，而荧光图像模拟的结构特征，在组织病理学。同时染料增强反射和荧光共聚焦显微镜显示的检测，划界和非侵入性监测皮肤癌的发展前景。
Skin cancer is the most common form of human cancer. Its early diagnosis and timely treatment is of paramount importance for dermatology and surgical oncology. In this study, we evaluate the use of reflectance and fluorescence confocal microscopy for detecting skin cancers in anin-vivotrial with B16F10 melanoma and SCCVII squamous cell carcinoma in mice. For the experiments, the mice are anesthetized, then the tumors are infiltrated with aqueous solution of methylene blue and imaged. Reflectance images are acquired at. Fluorescence is excited atand registered in the range between 690 and. After imaging, the mice are sacrificed. The tumors are excised and processed for hematoxylin and eosin histopathology, which is compared to the optical images. The results of the study indicate thatin-vivoreflectance images provide valuable information on vascularization of the tumor, whereas the fluorescence images mimic the structural features seen in histopathology. Simultaneous dye-enhanced reflectance and fluorescence confocal microscopy shows promise for the detection, demarcation, and noninvasive monitoring of skin cancer development.