Heterogeneous expression of the aquaporin 1 (AQP1) water channel in tumors of the prostate, breast, ovary, colon and lung: a study using high density multiple human tumor tissue microarrays.
Heterogeneous expression of the aquaporin 1 (AQP1) water channel in tumors of the prostate, breast, ovary, colon and lung: a study using high density multiple human tumor tissue microarrays.
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DOI：
10.3892/ijo.26.5.1149
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发表时间：
2005-05
影响因子：
5.2
通讯作者：
A. Mobasheri;R. Airley;S. Hewitt;D. Marples
中科院分区：
文献类型：
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作者：
A. Mobasheri;R. Airley;S. Hewitt;D. Marples
水通道蛋白1（AQP 1）水通道是一种膜蛋白，通过促进水穿过细胞膜来控制内皮和上皮屏障的渗透性。最近的研究表明，AQP1可能是脑、结肠、乳腺和胰腺肿瘤中高血管通透性和间质液压力的原因。AQP1还可能在肿瘤血管生成中发挥作用，并可能参与渗出液或水肿液的发展。本研究的目的是使用免疫组织化学和半定量组织形态学分析比较AQP1在NCI TARP人多肿瘤组织微阵列（TMAs）上的分布和相对丰度，并与CHTN TMAs上代表的正常组织进行比较。采用免疫组织化学和半定量组织形态学分析比较AQP1在TARP TMAs上的前列腺、结肠、肺、乳腺和卵巢肿瘤中的分布，并与CHTN TMAs上的正常肿瘤进行比较。AQP1在正常组织毛细血管内皮细胞均有表达。在大多数肿瘤中，AQP1仅限于内皮屏障。前列腺和卵巢肿瘤微血管结构中AQP1表达略高，晚期乳腺癌和结直肠癌中AQP1免疫反应性也见于某些肿瘤细胞中。总之，AQP 1水通道是微血管的一种出色标志物，但它在不同的人类肿瘤中异源表达，并且不一定在所有肿瘤细胞中表达。AQP1在某些人腺癌中的表达增加可能是血管生成的结果，对肿瘤水肿的形成或清除很重要。
Aquaporin 1 (AQP1) water channels are membrane proteins that control the permeability of endothelial and epithelial barriers by facilitating water movement across cell membranes. Recent studies suggest that AQP1 may be responsible for the high vascular permeability and interstitial fluid pressure in tumors of the brain, colon, breast and pancreas. AQP1 may also play a role in tumor angiogenesis and may be involved in development of effusions or edema fluid. The aim of the present study was to use immunohistochemistry and semi-quantitative histomorphometric analysis to compare the distribution and relative abundance of AQP1 on NCI TARP human multiple tumor tissue microarrays (TMAs) with normal tissues represented on the CHTN TMAs. Immunohistochemistry and semi-quantitative histomorphometric analysis were used to compare the distribution of AQP1 in tumors of the prostate, colon, lung, breast and ovary represented on TARP TMAs with their normal counterparts on CHTN TMAs. AQP1 was expressed in capillary endothelia of all normal tissues. In most tumors AQP1 was confined to endothelial barriers. AQP1 expression was marginally higher in microvascular structures in prostate and ovarian tumors and was higher in advanced mammary and colorectal carcinomas where AQP1 immunoreactivity was also seen in some neoplastic tumor cells. In conclusion, the AQP1 water channel is an excellent marker of microvasculature but it is heterogeneously expressed in different human tumors and not necessarily expressed in all neoplastic cells. Increased AQP1 expression in some human adenocarcinomas may be a consequence of angiogenesis and important for the formation or clearance of tumor edema.