Genetic heterogeneity among visceral and post-Kala-Azar dermal leishmaniasis strains from eastern India
Genetic heterogeneity among visceral and post-Kala-Azar dermal leishmaniasis strains from eastern India
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DOI：
10.1016/j.meegid.2006.09.001
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发表时间：
2007-03-01
影响因子：
3.2
通讯作者：
Singh, Sarman
中科院分区：
文献类型：
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作者：
Dey, Ayan;Singh, Sarman
在印度和苏丹，一些内脏利什曼病患者会发展为Kala-Azar后皮肤利什曼病(PKDL)，而大多数人不会。同样，据报道，印度的临床表现和治疗结果因地区和邦的不同而不同。本研究旨在找出VL和PKDL致病菌株之间的遗传变异。从18例内脏利什曼病患者和6例内脏利什曼病患者体内分离的24株杜氏利什曼原虫的核DNA经PstI限制性内切酶消化后，以地高辛标记的β-微管蛋白为探针进行Southern杂交。结果显示，18株VL菌株有3种不同的带型。然而，所有的PKDL分离株都表现出遗传上的同质性，而VL分离株则表现出异质性。有趣的是，在比哈尔邦发现了最多的异源类群，但所有来自西孟加拉邦的分离株都显示出单一的基因型来源。这项研究表明，基因突变可能是印度杜诺瓦尼乳杆菌内脏品系中PKDL变异和发展的原因。(C)2006爱思唯尔B.V.保留所有权利。
In India and Sudan, some patients of visceral leishmaniasis develop post-Kala-Azar dermal leishmaniasis (PKDL) while majority will not. Similarly, the clinical manifestations and treatment outcome are reported to vary from district to district and state to state in India. Present study is focused on to find out the genetic variations between VL & PKDL causing strains. Nuclear DNA from 24 strains of Leishmania donovani, isolated from patients of visceral leishmaniasis (18) and PKDL (6) was extracted and digested with PstI restriction enzyme followed by southern hybridization using Dig labeled beta-tubulin as probe. The results showed three different banding patterns among 18 VL strains. However, all PKDL isolates showed a genetic homogeneity within themselves but heterogeneity from VL isolates. Interestingly maximum heterogenic groups were found in Bihar but all isolates from West Bengal showed a single genotype origin. This study shows that genetic mutations might be responsible for such variation and development of PKDL in visceral strains of Indian L. donovani. (c) 2006 Elsevier B.V. All rights reserved.