Structural basis for Klf4 recognition of methylated DNA.
Structural basis for Klf4 recognition of methylated DNA.
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DOI：
10.1093/nar/gku134
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发表时间：
2014-04
影响因子：
14.9
通讯作者：
Cheng X
中科院分区：
文献类型：
--
作者：
Liu Y;Olanrewaju YO;Zheng Y;Hashimoto H;Blumenthal RM;Zhang X;Cheng X
Transcription factor Krüppel-like factor 4 (Klf4), one of the factors directing cellular reprogramming, recognizes the CpG dinucleotide (whether methylated or unmodified) within a specific G/C-rich sequence. The binding affinity of the mouse Klf4 DNA-binding domain for methylated DNA is only slightly stronger than that for an unmodified oligonucleotide. The structure of the C-terminal three Krüppel-like zinc fingers (ZnFs) of mouse Klf4, in complex with fully methylated DNA, was determined at 1.85 Å resolution. An arginine and a glutamate interact with the methyl group. By comparison with two other recently characterized structures of ZnF protein complexes with methylated DNA, we propose a common principle of recognition of methylated CpG by C2H2 ZnF proteins, which involves a spatially conserved Arg–Glu pair.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
7.7
作者：
Hu S;Wan J;Su Y;Song Q;Zeng Y;Nguyen HN;Shin J;Cox E;Rho HS;Woodard C;Xia S;Liu S;Lyu H;Ming GL;Wade H;Song H;Qian J;Zhu H
通讯作者：
Zhu H
影响因子：
13.8
作者：
Liu, Yiwei;Zhang, Xing;Blumenthal, Robert M.;Cheng, Xiaodong
通讯作者：
Cheng, Xiaodong
影响因子：
5.6
作者：
BESTOR, T;LAUDANO, A;INGRAM, V
通讯作者：
INGRAM, V