In vitro behaviour of endothelial cells on a titanium surface.
In vitro behaviour of endothelial cells on a titanium surface.
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DOI：
10.1186/1746-160x-4-14
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发表时间：
2008-07-23
影响因子：
3
通讯作者：
Kleinheinz J
中科院分区：
文献类型：
--
作者：
Breithaupt-Faloppa AC;de Lima WT;Oliveira-Filho RM;Kleinheinz J
内皮细胞在细胞递送至炎症部位、趋化性、细胞粘附和外渗方面发挥着重要作用。将异物植入人体会导致炎症和修复反应，涉及不同类型的细胞和释放的大量化学介质。除了分析细胞活力的维持之外，评估内皮细胞和植入材料的相互作用还必须考虑其他参数。在本研究中，我们使用组织学和免疫组织化学方法检查了在钛 (Ti) 上收获的人脐静脉内皮细胞 (HUVEC) 的行为。细胞传代两次后，以标准密度接种在商业板状钛片上，并维持1、7或14天。 14 天后，我们可以在钛表面观察到融合的单层内皮细胞 (EC)。 Ti/细胞接触一天后，纤连蛋白的表达主要在细胞质中，并且比对照表面上的表达更强。在与钛接触的细胞上，随着α5β1整合素的时间，观察到强烈且均匀的细胞表达。发现 EC 在钛上的附着与细胞来源的纤连蛋白及其特定受体 α5β1 整合素的结合有关。据观察，钛有效地充当内皮细胞附着、生长和增殖的合适基质。然而，在与 Ti 接触 7 天后，Weibel-Palade 小体似乎没有完全加工并表现出异常形态，PECAM-1 定位也发生相应改变。
Endothelial cells play an important role in the delivery of cells to the inflammation site, chemotaxis, cell adhesion and extravasation. Implantation of a foreign material into the human body determines inflammatory and repair reactions, involving different cell types with a plethora of released chemical mediators. The evaluation of the interaction of endothelial cells and implanted materials must take into account other parameters in addition to the analysis of maintenance of cell viability. In the present investigation, we examined the behavior of human umbilical vein endothelial cells (HUVECs) harvested on titanium (Ti), using histological and immunohistochemical methods. The cells, after two passages, were seeded in a standard density on commercially plate-shaped titanium pieces, and maintained for 1, 7 or 14 days. After 14 days, we could observe a confluent monolayer of endothelial cells (ECs) on the titanium surface. Upon one-day Ti/cell contact the expression of fibronectin was predominantly cytoplasmatic and stronger than on the control surface. It was observed strong and uniform cell expression along the time of α5β1 integrin on the cells in contact with titanium. The attachment of ECs on titanium was found to be related to cellular-derived fibronectin and the binding to its specific receptor, the α5β1 integrin. It was observed that titanium effectively serves as a suitable substrate for endothelial cell attachment, growth and proliferation. However, upon a 7-day contact with Ti, the Weibel-Palade bodies appeared to be not fully processed and exhibited an anomalous morphology, with corresponding alterations of PECAM-1 localization.