A synthetic peptide substrate for selective assay of protein kinase C.
A synthetic peptide substrate for selective assay of protein kinase C.
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DOI：
10.1016/0006-291x(90)90996-z
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发表时间：
1990-02
影响因子：
3.1
通讯作者：
I. Yasuda;A. Kishimoto;Shoichiro Tanaka;M. Tominaga;A. Sakurai;Y. Nishizuka
中科院分区：
文献类型：
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作者：
I. Yasuda;A. Kishimoto;Shoichiro Tanaka;M. Tominaga;A. Sakurai;Y. Nishizuka
在目前测试的各种磷酸受体蛋白和多肽中，具有髓鞘碱性蛋白Ser(8)序列的合成肽Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu，(MBP4-14)是最特异和最方便的底物，可以用于选择性地测定蛋白激酶C。该肽不被环磷酸腺苷依赖的蛋白激酶、酪蛋白激酶I和II、钙/钙调蛋白依赖的蛋白激酶II或磷酸化酶所磷酸化，可以常规地用于粗组织提取液中低背景的蛋白激酶C的测定。Km值相当低(7μM)，Vmax值是Hl组蛋白的两倍。
Among various phosphate acceptor proteins and peptides so far tested, a synthetic peptide having the sequence surrounding Ser (8) of myelin basic protein, Gln-Lys-Arg-Pro-Ser(8)-Gln-Arg-Ser-Lys-Tyr-Leu, (MBP4–14), is the most specific and convenient substrate which can be used for selective assay of protein kinase C. This peptide is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts. The Km value is considerably low (7 μM) with a Vmaxvalue of twice as much as that for Hl histone.