Serum-free primary culture of normal mouse mammary epithelial and stromal cells.
Serum-free primary culture of normal mouse mammary epithelial and stromal cells.
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正常小鼠乳腺上皮细胞和基质细胞的无血清原代培养物。
DOI：
10.1007/bf02639396
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Haslam,SZ
中科院分区：
文献类型：
--
作者：
Wang,S;Haslam,SZ
体外无血清培养系统为了解乳腺上皮细胞和成纤维细胞的局部激素调节提供了重要途径，避免了体内环境的复杂性和不确定血清因素的影响。已检查了单层培养物中上皮细胞、成纤维细胞以及上皮细胞和成纤维细胞组合的基底生长的基质条件和培养基组分。上皮细胞和混合细胞在补充有胎球蛋白和胰岛素的基本培养基中在胶原包被的表面上表现出良好的附着和维持。相反，富含成纤维细胞的培养物需要一个可塑的基质和一个补充有胰岛素、胎球蛋白和氢化可的松的培养基。在混合细胞培养中，成纤维细胞在支持上皮细胞维持的基本培养基中维持良好。这些结果表明，混合细胞培养物中上皮细胞的存在可以影响成纤维细胞功能。本研究开发的培养基可用于未来研究成纤维细胞和上皮细胞相互作用，涉及激素和生长因子对其生长和分化的调节。
An in vitro serum-free culture system provides an important approach to the understanding of local hormonal regulation of mammary epithelial and fibroblast cells, avoiding the complexity of the in vivo environment and the influence of undefined serum factors. The substratum conditions and medium components have been examined for the basal growth of epithelial cells, fibroblasts, and combined epithelial and fibroblast cells in monolayer cultures. Epithelial cells and mixed cells exhibit good attachment and maintenance on a collagen-coated surface in a minimal medium supplemented with fetuin and insulin. In contrast, fibroblast-enriched cultures require a plastic substratum and a medium supplemented with insulin, fetuin, and hydrocortisone. In mixed cell culture, fibroblasts are maintained well in the minimal media which supports the maintenance of epithelial cells. These results indicate that the presence of epithelial cells in mixed cell cultures can influence fibroblast function. The media developed in the present study can be used in future studies of fibroblast and epithelial cell interactions with regard to hormone and growth factor regulation of their growth and differentiation.