Shock wave induced cytoskeletal and morphological deformations in a human renal carcinoma cell line
Shock wave induced cytoskeletal and morphological deformations in a human renal carcinoma cell line
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DOI：
10.1111/j.1349-7006.2006.00172.x
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发表时间：
2006-04-01
期刊：
影响因子：
5.7
通讯作者：
Takayama, K
中科院分区：
文献类型：
--
作者：
Moosavi-Nejad, SF;Hosseini, SHR;Takayama, K
体外研究了冲击波对人肾癌细胞系（ACHN）形态和细胞骨架的影响。在盖玻片上培养的 ACHN 单层细胞经过 10 次聚焦水下冲击波处理，压电陶瓷冲击波发生器焦点区域的峰值压力为 16 MPa。暴露于冲击波后，根据处理细胞形态变形的严重程度，将单层分为三个形态区域；焦点、边缘和完整。发现形态变形与细胞内细胞骨架丝的紊乱有关。根据细胞在三个形态区域内的位置，分别研究了处理细胞中细胞骨架蛋白的变形。在三种主要的细胞骨架蛋白中，肌动蛋白和微管蛋白，但波形蛋白不受冲击波影响。变形细胞在 3 小时内重组其细胞骨架网络，其模式与对照相似，表明冲击波在存活细胞中诱导细胞骨架损伤的瞬时特征。载玻片上剩余的细胞碎片含有短肌动蛋白丝，表明剪切应力在冲击波破坏细胞骨架纤维中的重要作用。
Effects of shock waves on the morphology and cytoskeleton of a human renal carcinoma cell line (ACHN) were investigated in vitro. ACHN monolayer cultured on a cover slide glass was treated with 10 shots of focused underwater shock waves, with 16 MPa peak pressure at the focal area of a piezoceramic shock wave generator. After exposure to the shock wave, based on the severity of morphological deformations of the treated cells, the monolayer was divided into three morphological areas; focal, marginal and intact. Morphological deformations were found to be associated with disorganization of the intracellular cytoskeletal filaments. Deformation of the cytoskeletal proteins in the treated cells were separately studied with respect to the location of the cells within the three morphological areas. Among three major cytoskeletal proteins, actin and tubulin, but not vimentin, were affected by the shock waves. The deformed cells reorganized their cytoskeletal network within 3 h with a pattern similar to the control, indicating the transient characteristic of the shock wave induced cytoskeletal damage in the surviving cells. The remaining cell fragments on the slide glass, which contained short actin filaments, indicated the important role of shear stress in damaging the cytoskeletal fibers by shock waves.