Acacetin-induced cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells
Acacetin-induced cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells
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DOI：
10.1016/j.canlet.2004.02.019
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发表时间：
2004-08-20
期刊：
影响因子：
9.7
通讯作者：
Lin, CC
中科院分区：
文献类型：
--
作者：
Hsu, YL;Kuo, PL;Lin, CC
在这项研究中，我们检测了金合欢素(5，7-二羟基-4-甲氧基黄酮)，一种黄酮类化合物，以观察其对人非小细胞肺癌A549细胞增殖的影响。首次报道了金合欢素不仅能抑制A549细胞的增殖，还能诱导细胞凋亡，并将细胞周期进程阻断在G1期。ELISA法检测结果显示，金合欢素可显著增加细胞中P53和p21/WAF1蛋白的表达，导致细胞周期停滞。金合欢素诱导的细胞凋亡可能与Fas及其两种配体--膜结合型Fas配体(MFasL)和可溶性Fas配体(SFasL)的表达增强有关。综上所述，P53和Fas/FasL凋亡系统可能参与了金合欢素对A549细胞的抗增殖作用。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
In this study, we examined acacetin (5,7-dihydroxy-4-methoxyflavone), a flavonoid compound, for its effect on proliferation in human non-small cell lung cancer A549 cells. The results first reported that acacetin not only inhibited A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. ELISA assay demonstrated that acacetin significantly increased the expression of p53 and p21/WAF1 protein, which caused cell cycle arrest. An enhancement in Fas and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), might be responsible for the apoptotic effect induced by acacetin. Taken together, p53 and Fas/FasL apoptotic system may participate in the antiproliferative activity of acacetin in A549 cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved.