Identification of a cullin homology region in a subunit of the anaphase-promoting complex.
Identification of a cullin homology region in a subunit of the anaphase-promoting complex.
复制标题
DOI：
10.1126/science.279.5354.1219
复制
发表时间：
1998-02
期刊：
影响因子：
56.9
通讯作者：
Hongtao Yu;Jan-Michael Peters;Randall W. King;A. M. Page;Philip Hieter;Marc W. Kirschner
中科院分区：
文献类型：
--
作者：
Hongtao Yu;Jan-Michael Peters;Randall W. King;A. M. Page;Philip Hieter;Marc W. Kirschner
后期促进复合物由八个蛋白亚基组成，包括BimE（APC 1）、CDC 27（APC 3）、CDC 16（APC 6）和CDC 23（APC 8）。克隆了剩余的四个人APC亚基，APC 2、APC 4、APC 5和APC 7，以及人CDC 23。APC 7含有多个拷贝的四肽重复序列，类似于CDC 16、CDC 23和CDC 27。APC 4和APC 5与已知功能的蛋白质没有相似性，而APC 2含有与cullin中的序列相似的区域，cullin是一个涉及G1期细胞周期蛋白和细胞周期蛋白依赖性激酶抑制剂泛素化的蛋白质家族。APC 2基因在酿酒酵母中是必需的，并且apc 2突变体在中期停滞并且在Pds1p的降解中有缺陷。APC 2和cullin可能是泛素连接酶家族的远亲成员，其靶向细胞周期调节剂进行降解。
The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratrico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.