Automatic microtubule tracking for QD-based in vivo cell imaging and drug efficacy study.
Automatic microtubule tracking for QD-based in vivo cell imaging and drug efficacy study.
复制标题
用于基于 QD 的体内细胞成像和药效研究的自动微管跟踪。
DOI：
10.1109/iembs.2006.259750
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Wang,MayD
中科院分区：
文献类型：
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作者：
Kong,KoonYin;Marcus,AdamI;Hong,JinYoung;Giannakakou,Paraskevi;Wang,MayD
微管（MT）是一种动态聚合物，可以在生长、缩短和暂停状态之间快速转换。这些动态事件对于许多微管功能如细胞内运输和信号传导至关重要。此外，靶向微管的癌症化疗药物，如紫杉烷和长春花生物碱，已知在低剂量下抑制微管动力学，导致有丝分裂停滞和细胞死亡。微管动力学的定量可用作抗癌药物活性的读数，并且可以是药物敏感性/耐药性的替代标志物。新兴的纳米技术，如量子点，提供了诸如更少的光漂白，更高的探针成像强度，更好的特异性和灵敏度等特性，最终使得在足够长的时间内可视化亚细胞事件成为可能。但这也导致了数据采集量的大幅增加。手工注释机器翻译的传统方式正在成为一项艰巨的任务。因此，研究和开发一种高效、可靠、快速的MT跟踪方法是本文的目标。在本文中，我们描述了主动轮廓为基础的跟踪方法来自动跟踪MT。我们专门为开放蛇重新定义了内部能量项，并检查了用于定位微管末端的不同外部能量项。该算法已经使用模拟图像、未处理的MCF-7乳腺癌细胞的图像和用微管靶向化疗剂Taxol处理的细胞的图像进行了验证
Microtubules (MT) are dynamic polymers that rapidly transition between states of growth, shortening, and pause. These dynamic events are critical for many microtubule functions such as intracellular trafficking and signaling. In addition, cancer chemotherapy drugs that target microtubules, such as the taxanes and the vinca alkaloids, are known to suppress microtubule dynamics at low doses, leading to mitotic arrest and cell death. Quantification of microtubule dynamics can be used as a read-out of anticancer-drug activity and can be a surrogate marker of drug sensitivity/resistance. The emerging nanotechnology such as quantum dots has provided properties such as less photo bleaching, higher probe imaging intensity, better specificity and sensitivity, which finally makes visualizing subcellular events over long enough time a possibility. But it also results in big increase in data acquisition. The traditional way of annotating MT manually is becoming a daunting task. Thus, the goal is to research and develop an efficient, reliable, and rapid MT tracking. In this paper, we describe active contour-based tracking methods to automatically track MT. We redefine the internal energy terms specifically for open snake, and examine different external energy terms for locating the end tips of a microtubule. This algorithm has been validated using simulated images, images of untreated MCF-7 breast cancer cells, and image of cells treated with the microtubule-targeting chemotherapeutic agent, Taxol