Cell-Surface Engineering by a Conjugation-and-Release Approach Based on the Formation and Cleavage of Oxime Linkages upon Mild Electrochemical Oxidation and Reduction
Cell-Surface Engineering by a Conjugation-and-Release Approach Based on the Formation and Cleavage of Oxime Linkages upon Mild Electrochemical Oxidation and Reduction
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DOI：
10.1002/anie.201404099
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发表时间：
2014-09-01
影响因子：
16.6
通讯作者：
Yousaf, Muhammad N.
中科院分区：
文献类型：
--
作者：
Pulsipher, Abigail;Dutta, Debjit;Yousaf, Muhammad N.
我们报告了一种重新连接细胞表面的策略，用于动态控制细胞膜上的配体组成和调节细胞-细胞相互作用，以产生三维（3D）组织结构，应用于干细胞分化，细胞表面剪裁和组织工程。我们在脂质体融合和递送方法的基础上，用生物正交化学基团定制细胞表面，通过化学选择结合和释放，创建动态、电活性和可切换的细胞组织组件。修饰细胞表面的每一步：活化、结合、释放和再生，都可以通过无创、无标记的分析技术进行监测和调节。我们通过小分子在细胞表面的结合和释放，以及3D共培养球体和多层细胞组织的生成，证明了这种方法的实用性，这些细胞组织可以根据需要进行编程组装和拆卸。
We report a strategy to rewire cell surfaces for the dynamic control of ligand composition on cell membranes and the modulation of cell-cell interactions to generate three-dimensional (3D) tissue structures applied to stem-cell differentiation, cell-surface tailoring, and tissue engineering. We tailored cell surfaces with bioorthogonal chemical groups on the basis of a liposome-fusion and -delivery method to create dynamic, electroactive, and switchable cell-tissue assemblies through chemistry involving chemoselective conjugation and release. Each step to modify the cell surface: activation, conjugation, release, and regeneration, can be monitored and modulated by noninvasive, label-free analytical techniques. We demonstrate the utility of this methodology by the conjugation and release of small molecules to and from cell surfaces and by the generation of 3D coculture spheroids and multilayered cell tissues that can be programmed to undergo assembly and disassembly on demand.