Investigating membrane protein dynamics in living cells
Investigating membrane protein dynamics in living cells
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DOI：
10.1139/o06-189
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发表时间：
2006-12-01
影响因子：
2.9
通讯作者：
Hanrahan, John W.
中科院分区：
文献类型：
--
作者：
Bates, Ian R.;Wiseman, Paul W.;Hanrahan, John W.
活细胞成像是了解膜蛋白功能和调控的有力工具。本文综述了基于荧光显微镜研究膜蛋白转运动力学的4种技术：荧光相关光谱技术、图像相关光谱技术、光漂白后荧光恢复技术、单粒子和（或）分子跟踪技术。每种方法的优点和局限性都用离子通道和细胞粘附分子的最新研究来说明。
Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of mernbrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.