Probiotic Bifidobacterium animalis subsp. lactis Bi-07 alleviates bacterial translocation and ameliorates microinflammation in experimental uraemia.
Probiotic Bifidobacterium animalis subsp. lactis Bi-07 alleviates bacterial translocation and ameliorates microinflammation in experimental uraemia.
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益生菌动物双歧杆菌亚种
DOI：
10.1111/nep.12272
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
冯婕
中科院分区：
文献类型：
--
作者：
魏萌;王志刚;刘华;蒋红利;王萌;梁珊珊;史珂慧;冯婕
AimUraemia is characterized by intestinal bacterial translocation, which contributes to the development of microinflammation. Probiotics enhance the intestinal barrier and overall health of the host. The present study investigated whether the probioticBifidobacterium animalissubsp.lactisBi‐07 alleviates bacterial translocation and ameliorates microinflammation in experimental uraemia.MethodsSixty Sprague–Dawley rats were divided into three groups of 20 rats each: the sham group, which underwent only laparotomy; the uraemia group, which underwent 5/6 nephrectomy; and the uraemia + probiotic group, which underwent 5/6 nephrectomy and daily intragastric administration ofB. animalissubsp.lactisBi‐07 for 4 weeks. Bacterial translocation was evaluated by polymerase chain reaction amplification of the green fluorescent protein (GFP) gene from oral GFP‐labelledEscherichia coliin the peripheral blood, mesenteric lymph nodes, liver, and spleen. Intestinal permeability, plasma inflammatory biomarker levels, and endotoxin levels were measured. Jejunum, ileum, and colon specimens were removed for histological examination.ResultsUraemic rats exhibited a significantly higher incidence of bacterial translocation (70%) than did sham rats (10%). Probiotic treatment resulted in a decrease in bacterial translocation (20%). Intestinal permeability, inflammatory biomarker levels, and endotoxin levels in uraemic rats were significantly higher than those in the sham group. After treatment with the probiotic, inflammatory biomarker levels significantly decreased. Uraemic rats demonstrated superficial mucosal erosion and inflammatory cell infiltration in the small intestine, and administration of the probiotic alleviated these lesions.ConclusionThe probioticB. animalissubsp.lactisBi‐07 alleviate bacterial translocation and ameliorate microinflammation through the recovery of intestinal mucosal integrity.
