Molecular cloning of macrophin, a human homologue of Drosophila kakapo with a close structural similarity to plectin and dystrophin.
Molecular cloning of macrophin, a human homologue of Drosophila kakapo with a close structural similarity to plectin and dystrophin.
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DOI：
10.1006/bbrc.1999.1538
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发表时间：
1999-10
影响因子：
3.1
通讯作者：
T. Okuda;S. Matsuda;S. Nakatsugawa;Y. Ichigotani;N. Iwahashi;M. Takahashi;T. Ishigaki;M. Hamaguchi-M.-Hamag
中科院分区：
文献类型：
--
作者：
T. Okuda;S. Matsuda;S. Nakatsugawa;Y. Ichigotani;N. Iwahashi;M. Takahashi;T. Ishigaki;M. Hamaguchi-M.-Hamag
我们通过退化引物介导的PCR策略确定了一种新型细胞骨架蛋白的完整cDNA编码序列。这个新基因被命名为巨噬蛋白（微丝和肌动蛋白丝）交联蛋白，与粘连蛋白和肌营养不良蛋白相关。AB029290)，似乎是果蝇基因kakapo的人类同源物，并且在BLAST同源性计算数据库中搜索显示与plectin和dystrophin非常相似。巨噬蛋白与鸮鹦鹉的蛋白质序列相似度为66%，均具有nh2末端的肌动蛋白结合域、由谱蛋白样重复序列组成的中心棒状区域和cooh末端的gas2相关区域。预测的巨噬蛋白序列长度为5430个氨基酸，计算的分子质量为620 kDa，是人类细胞骨架蛋白中最大的序列之一。巨噬蛋白在脑、心、肺、胎盘、肝、肾、胰腺中均有高表达。在胰腺中，我们通过原位杂交发现巨噬蛋白在腺泡细胞中特异性表达，而在胰岛细胞中特异性表达。通过辐射杂交面板，我们将巨噬蛋白基因定位到染色体1p31-32上。
We have determined the complete cDNA coding sequence of a novel cytoskeletal protein by the degenerative primer-mediated PCR strategy. This novel gene, named as macrophin (microfilament and actin filament cross-linker protein related to plectin and dystrophin, Accession No. AB029290), appears to be a human homologue of a Drosophila gene, kakapo, and shows close similarity to plectin and dystrophin on the search of BLAST homology-computed database. Comparison of the deduced protein sequences for macrophin and kakapo revealed that they were 66% similar, and both of them have an NH2-terminal actin-binding domain, a central rod region composed of spectrin-like repeats, and COOH-terminal Gas2-related region. The predicted sequences for macrophin are 5430 amino acids in length with a calculated molecular mass of 620 kDa, which is one of the largest size idenfied in human cytoskeletal proteins. High expression of macrophin was observed in brain, heart, lung, placenta, liver, kidney, and pancreas. In pancreas, we found that macrophin was specifically expressed in acinar cells than islet cells by in situ hybridization. By using radiation hybrid panel, we have mapped the macrophin gene to the chromosome 1p31-32.