Translocations t(11;18)(q21;q21) and t(14;18)(q32;q21) are the main chromosomal abnormalities involving MLT/MALT1 in MALT lymphomas-猫眼课题宝

易位 t(11;18)(q21;q21) 和 t(14;18)(q32;q21) 是 MALT 淋巴瘤中涉及 MLT/MALT1 的主要染色体异常

DOI：

10.1038/sj.leu.2403122

发表时间：

2003

期刊：

Leukemia

影响因子：

11.4

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

E. Penas;K. Hinz;K. Röser;C. Copie;I. Wlodarska;P. Marynen;A. Hagemeijer;P. Gaulard;T. Löning;D. Hossfeld;J. Dierlamm

最近发现的MLT/MALT1基因与t(11;18)(q21;q21)中的API2基因融合，这是大约三分之一的MALT淋巴瘤的特征。为了筛选 MLT/MALT1 的变异易位和扩增，我们开发了一种新型非定向双色间期荧光原位杂交 (FISH) 检测，其中使用 MLT/MALT1 侧翼的两个 PAC 克隆。该测定适用于 108 例边缘区 B 细胞淋巴瘤 (MZBCL)，包括 72 例结外 MALT 淋巴瘤、17 例淋巴结和 19 例脾 MZBCL。在 19 例 MALT 淋巴瘤 (26%) 中，但在淋巴结或脾 MZBCL 中均未发现 MLT/MALT1 侧翼探针的分离杂交信号。进一步的 FISH 分析显示，这 19 例中有 12 例显示经典的 t(11;18)，其余 7 例显示新型 t(14;18)(q32;q21)，涉及 MLT/MALT1 和 IGH 基因。这些易位发生的频率随疾病的主要部位而显着变化。 t(11;18)主要在胃肠道MALT淋巴瘤中检测到，而t(14;18)出现在腮腺和结膜MALT淋巴瘤中。在任何分析的淋巴瘤中均未观察到 MLT/MALT1 扩增。我们得出结论，易位 t(11;18)(q21;q21) 和 t(14;18)(q21;q32) 代表 MALT 淋巴瘤中涉及 MLT/MALT1 的主要结构畸变，而 MLT/MALT1 的真正扩增很少发生在 MZBCL 中。

The recently discovered MLT/MALT1 gene is fused with the API2 gene in the t(11;18)(q21;q21), which characterizes about one-third of MALT lymphomas. In order to screen for variant translocations and amplifications of MLT/MALT1, we have developed a novel, undirected two-color interphase fluorescence in situ hybridization (FISH) assay with two PAC clones flanking MLT/MALT1. This assay was applied to 108 marginal zone B-cell lymphomas (MZBCLs), including 72 extranodal MALT lymphomas, 17 nodal, and 19 splenic MZBCL. In 19 MALT lymphomas (26%), but in none of the nodal or splenic MZBCL, separated hybridization signals of the MLT/MALT1 flanking probes, were found. Further FISH analyses showed that 12 of these 19 cases displayed the classical t(11;18) and the remaining seven cases revealed the novel t(14;18)(q32;q21), involving the MLT/MALT1 and IGH genes. The frequency at which these translocations occurred varied significantly with the primary location of disease. The t(11;18) was mainly detected in gastrointestinal MALT lymphomas, whereas the t(14;18) occurred in MALT lymphomas of the parotid gland and the conjunctiva. Amplification of MLT/MALT1 was not observed in any of the lymphomas analyzed. We conclude that the translocations t(11;18)(q21;q21) and t(14;18)(q21;q32) represent the main structural aberrations involving MLT/MALT1 in MALT lymphomas, whereas true amplifications of MLT/MALT1 occur rarely in MZBCL.

ERBB2 AMPLIFICATION IN BREAST-CANCER ANALYZED BY FLUORESCENCE INSITU HYBRIDIZATION

DOI： 10.1073/pnas.89.12.5321

发表时间： 1992-06-15

期刊：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

影响因子： 11.1

作者：

KALLIONIEMI, OP;KALLIONIEMI, A;GRAY, JW

通讯作者：

GRAY, JW

Meiotic recombination between yeast artificial chromosomes yields a single clone containing the entire BCL2 protooncogene.