Biodegradation of keratinous waste by Chryseobacterium sp RBT isolated from soil contaminated with poultry waste
Biodegradation of keratinous waste by Chryseobacterium sp RBT isolated from soil contaminated with poultry waste
复制标题
DOI：
10.1002/jobm.201100371
复制
发表时间：
2013-02-01
影响因子：
3.1
通讯作者：
Jadhav, Jyoti P.
中科院分区：
文献类型：
--
作者：
Gurav, Ranjit G.;Jadhav, Jyoti P.
在本研究中，从印度科哈普尔的家禽废弃物处理场分离到一株羽毛降解菌。经鉴定，该菌为金杆菌属。RBT采用16S rRNA基因序列分析。金杆菌属。RBT在30h内迅速降解天然羽毛，并产生最高水平的角蛋白水解酶活性(98.3U/ml)。含有角蛋白的废物，即。对丝绸、人发、羊毛和鸡毛进行了角蛋白降解能力的测定。在所测试的底物中，金黄色杆菌属。RBT对鸡毛具有更高的特异性(98.6%降解率)，最大角蛋白酶活力(98.3U/ml)和可溶性蛋白浓度(3.84 mg/ml)。考察了不同物理化学参数(温度、pH、碳源和氮源)对角蛋白水解酶产量的影响。在pH(8.6)和温度(50℃)下，角蛋白水解酶活性最高。糖蜜(1.0%w/v)作为诱导剂，可使角蛋白分解活性提高一倍，而淀粉则起抑制作用。用粗制角蛋白酶(2%v/v)处理山羊皮肤，12h内脱毛完全。因此，将其命名为金杆菌属。RBT在以环保的方式处理角质废物方面显示出潜力。
In the present study, a feather degrading bacterial strain was isolated from poultry waste disposal site, Kolhapur, India. The bacterium was identified as Chryseobacterium sp. RBT using 16S rRNA gene sequence analysis. Chryseobacterium sp. RBT showed rapid hydrolysis of native feathers within 30h and produced the highest level of keratinase activity (98.3U/ml). Keratin containing wastes viz. silk, human hair, wool and chicken feathers were tested for keratin degrading ability of the bacterium. Amongst the tested substrates, the Chryseobacterium sp. RBT showed more specificity towards chicken feathers (98.6% degradation) with maximum keratinase activity (98.3U/ml) and solubilized protein concentration (3.84mg/ml). Effect of various physico-chemical parameters (temperature, pH, carbon and nitrogen sources) on keratinase production was monitored. The maximum keratinase activity was observed at pH (8.6) and temperature (50 degrees C). Molasses (1.0%w/v) acted as an inducer and enhanced the keratinolytic activity by two fold, while starch worked as an inhibitor. The goat skin when treated with crude keratinase enzyme (2% v/v), showed complete dehairing within 12h. Hence, Chryseobacterium sp. RBT shows potential as a candidate for treating the keratinous waste in an ecofriendly manner.