The YmdB Phosphodiesterase Is a Global Regulator of Late Adaptive Responses in Bacillus subtilis
The YmdB Phosphodiesterase Is a Global Regulator of Late Adaptive Responses in Bacillus subtilis
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DOI：
10.1128/jb.00826-13
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发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
Stuelke, Joerg
中科院分区：
文献类型：
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作者：
Diethmaier, Christine;Newman, Joseph A.;Stuelke, Joerg
缺乏ymdB的枯草芽孢杆菌突变体不能形成生物膜，表现出鞭毛蛋白基因hag的强烈过表达，并且缺乏SinR拮抗剂SlrR。在这里，我们报告了YmdB的功能和结构特征，我们发现YmdB是一种磷酸二酯酶，对2',3'-和3',5'-环核苷酸单磷酸具有活性。YmdB的结构表明该酶采用具有双核金属中心的保守磷酸二酯酶折叠。催化关键残基的诱变表明，YmdB的酶活性对生物膜的形成至关重要。ymdB的缺失影响了800多个基因的表达；sigma(D)依赖的运动调控基因和几个产孢基因的水平升高，而sinr抑制的生物膜基因的水平降低，证实了YmdB在调节枯草芽孢杆菌后期适应性反应中的作用。
Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2', 3'- and 3', 5'-cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the sigma(D)-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis.