Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells.
Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells.
复制标题
结构稳定的分生植物细胞中有组织的微管阵列的免疫荧光显微镜。
DOI：
10.1083/jcb.89.3.685
复制
发表时间：
1981-06
影响因子：
7.8
通讯作者：
Gunning, B E
中科院分区：
文献类型：
--
作者：
Wick, S M;Seagull, R W;Osborn, M;Weber, K;Gunning, B E
在多细胞根尖的多聚甲醛固定和在细胞壁消化酶中短暂孵育后，制备细胞用于间接免疫荧光显微镜检查。这允许随后将组织分离成单个细胞或短的细胞文件，将其置于用聚赖氨酸包被的盖玻片上。与由活组织制成的球形原生质体不同，这些制备物保留了与它们所来源的细胞相同的多面体形状。细胞内容物，包括有组织的微管阵列，同样在结构上稳定。猪脑微管蛋白的抗体与已知存在于植物分生组织细胞中的所有类型的微管阵列反应，即，间期皮质微管、前前期带、有丝分裂纺锤体和成膜体微管。保留在透化，分离，稳定的细胞从典型的，壁封闭的植物细胞的抗原性有很大的潜力，植物免疫细胞化学，特别是应该促进工作的微管在有组织的植物组织的形态发生中的作用。
Cells were prepared for indirect immunofluorescence microscopy after paraformaldehyde fixation of multicellular root apices and brief incubation in cell wall-digesting enzymes. This allowed subsequent separation of the tissue into individual cells or short files of cells which were put onto coverslips coated with polylysine. Unlike spherical protoplasts made from living tissues, these preparations retain the same polyhedral shape as the cells from which they are derived. Cellular contents, including organized arrays of microtubules, are likewise structurally stabilized. Antibodies to porcine brain tubulin react with all types of microtubule array known to occur in plant meristematic cells, namely, interphase cortical microtubules, pre- prophase bands, the mitotic spindle, and phragmoplast microtubules. The retention of antigenicity in permeabilized, isolated, stabilized cells from typical, wall-enclosed plant cells has much potential for plant immunocytochemistry, and in particular should facilitate work on the role of microtubules in the morphogenesis of organized plant tissues.