FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE)
FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE)
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苜蓿臭虫 Adelphocoris lineolatus (GOEZE) 中气味结合蛋白 1 的功能特征和免疫定位
DOI：
10.1002/arch.20427
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发表时间：
2011-06-01
影响因子：
2.2
通讯作者：
Zhang, Yong-Jun
中科院分区：
文献类型：
--
作者：
Gu, Shao-Hua;Wang, Wei-Xuan;Zhang, Yong-Jun
在昆虫门中，个体与其环境之间的关系通常通过化学通讯来调节。气味结合蛋白（OBP）在昆虫嗅觉器官中广泛而稳定地表达，在化学传感和将疏水性气味剂穿过感器淋巴运输到嗅觉受体神经元中发挥着关键作用。在这项研究中，在苜蓿 Adelphocoris lineolatus 中发现、克隆并表达了一个新的 OBP 基因 (AlinOBP1)。实时PCR结果表明，AlinOBP1基因在各个发育阶段（从一龄到成虫）的表达水平有所不同，并且主要在成虫的触角中表达。 AlinOBP1在雄性触角中的表达水平是雌性触角的1.91倍。使用荧光探针 N-苯基-1-萘胺 (1-NPN) 通过荧光竞争性结合测量 AlinOBP1 与 114 种气味剂的结合特性。结果表明，AlinOBP1 与两种主要的假定信息素成分（丁酸乙酯和反式-2-己烯基丁酸酯）表现出较高的结合能力。此外，还观察到棉花释放的六种挥发物：辛醛、壬醛、癸醛、2-乙基-1-己醇、β-石竹烯和β-紫罗兰酮也与AlinOBP1结合。免疫细胞化学分析显示AlinOBP1在毛感器和基感器的感器淋巴中表达。我们的结果表明，AlinOBP1 可能在苜蓿的化学感知中充当载体。 (C) 2011 年 Wiley 期刊公司。
In the insect phylum, the relationships between individuals and their environment are often modulated by chemical communication. Odorant binding proteins (OBPs) are widely and robustly expressed in insect olfactory organs and play a key role in chemosensing and transporting hydrophobic odorants across the sensillum lymph to the olfactory receptor neuron. In this study, a novel OBP gene (AlinOBP1) in the lucerne plant bug, Adelphocoris lineolatus was identified, cloned and expressed. Real-time PCR results indicated that the expression level of AlinOBP1 gene differed in each developmental stage (from first instar to adult) and was predominantly expressed in the antennae of adults. The expression level of AlinOBP1 was 1.91 times higher in male antennae than in female antennae. The binding properties of AlinOBP1 with 114 odorants were measured using a fluorescence probe, N-phenyl-1-naphthylamine (1-NPN), with fluorescence competitive binding. The results revealed that AlinOBP1 exhibits high binding abilities with two major putative pheromone components, ethyl butyrate and trans-2-hexenyl butyrate. In addition, it was observed that six volatiles released from cotton, octanal, nonanal, decanal, 2-ethyl-1-hexanol, beta-caryophyllene and beta-ionone also bind to AlinOBP1. Immunocytochemistry analysis showed that AlinOBP1 was expressed in the sensillum lymph of sensilla trichodica and sensilla basiconca. Our results demonstrate that AlinOBP1 may function as a carrier in the chemoperception of the lucerne plant bug. (C) 2011 Wiley Periodicals, Inc.