7 DAYS OF BED REST DECREASE INSULIN ACTION ON GLUCOSE-UPTAKE IN LEG AND WHOLE-BODY
7 DAYS OF BED REST DECREASE INSULIN ACTION ON GLUCOSE-UPTAKE IN LEG AND WHOLE-BODY
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DOI：
10.1152/jappl.1991.70.3.1245
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发表时间：
1991-03-01
影响因子：
3.3
通讯作者：
GALBO, H
中科院分区：
文献类型：
--
作者：
MIKINES, KJ;RICHTER, EA;GALBO, H
在短期卧床休息后，正常人会出现糖耐量受损。为探讨胰岛素对全身葡萄糖摄取率(WBGUR)和腿部葡萄糖摄取率(LGUR)的影响机制，采用序贯正糖钳夹技术，结合股动脉和股静脉插管，在胰岛素浓度为10+/-1、18+/-1、37+/-2和360+/-15mU/ml的条件下，分别在卧床前(C)和卧床后(BR)7d测定胰岛素对全身葡萄糖摄取率(WBGUR)和腿部葡萄糖摄取率(LGUR)的影响。卧床后的WBGUR显著低于卧床前(分别为5.5+/-0.4和7.2+/-0.8 mg·min-1·kg-1)，卧床对LGUR的抑制更为明显，分别为0.6+/-0.1、0.9+/-0.2和2.8+/-0.4 mg·min-1腿-1(BR)，分别为0.9+/-0.1、1.7+/-0.4。和5.9+/-0.5 mg·min-1 kg腿-1(C)(P<；0.05)在三个较低的胰岛素浓度。同样，在这些胰岛素浓度下，通过间接量热法估计的乳酸释放、葡萄糖氧化和糖原储存在卧床后的腿部较低。在最高胰岛素剂量时，BR和C天的WBGUR相似，而卧床后LGUR较低。总而言之，卧床休息7天会减少全身的胰岛素作用，这一事实是由非活动肌肉中胰岛素作用的减少所解释的。
Impaired glucose tolerance develops in normal humans after short-term bed rest. To elucidate the mechanism, insulin action on whole body glucose uptake rate (WBGUR) and leg glucose uptake rate (LGUR) was measured by sequential euglycemic clamp technique combined with femoral arterial and venous cannulation at insulin concentrations of 10 +/- 1, 18 +/- 1, 37 +/- 2, and 360 +/- 15-mu-U/ml. Studies were performed before (C) and after (BR) 7 days of strict bed rest. WBGUR was significantly lower after bed rest than before (5.5 +/- 0.4 and 7.2 +/- 0.8 mg.min-1.kg-1, respectively) when insulin was 37-mu-U/ml. LGUR was even more markedly depressed by bed rest, being 0.6 +/- 0.1, 0.9 +/- 0.2, and 2.8 +/- 0.4 mg.min-1.kg leg-1 (BR) compared with 0.9 +/- 0.1, 1.7 +/- 0.4, and 5.9 +/- 0.5 mg.min-1.kg leg-1 (C) (P < 0.05) at the three lower insulin concentrations. At these insulin concentrations also, lactate release and glucose oxidation and glycogen storage estimated by indirect calorimetry were lower in the leg after bed rest. At the highest insulin dose WBGUR was similar on BR and C days, while LGUR was lower after bed rest. In conclusion, 7 days of bed rest decrease whole body insulin action, a fact that is explained by decreased insulin action in inactive muscle.