PHOTOREACTIVATION OF PYRIMIDINE DIMERS IN DNA FROM THYROID CELLS OF THE TELEOST, POECILIA FORMOSA
PHOTOREACTIVATION OF PYRIMIDINE DIMERS IN DNA FROM THYROID CELLS OF THE TELEOST, POECILIA FORMOSA
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硬骨鱼 POECILIA FORMOSA 甲状腺细胞 DNA 中嘧啶二聚体的光活化
DOI：
10.1111/j.1751-1097.1979.tb07053.x
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发表时间：
1979
影响因子：
3.3
通讯作者：
R. Setlow
中科院分区：
文献类型：
--
作者：
P. M. Achey;A. Woodhead;R. Setlow
摘要：我们开发并使用了一种简单的技术来估计未标记细胞 DNA 中嘧啶二聚体的数量。从细胞中提取 DNA，用二聚体特异性核酸内切酶处理，并通过其在碱性琼脂糖平板凝胶上的电泳迁移率估算其分子量。该技术用于证明来自 Poecilia formosa 鱼甲状腺组织的细胞对细胞 DNA 中的二聚体具有光再激活活性。
Abstract— We have developed and used a simple technique to estimate the quantity of pyrimidine dimers in unlabeled cellular DNA. DNA is extracted from cells, treated with an endonuclease specific for dimers, and its molecular weight estimated by its electrophoretic mobility on alkaline agarose slab gels. The technique is used to show that cells from thyroid tissue of the fish Poecilia formosa have photoreactivating activity towards dimers in the cellular DNA.