ACETYLATION OF SPERMIDINE AND SPERMINE BY RAT-LIVER AND KIDNEY CHROMATIN
ACETYLATION OF SPERMIDINE AND SPERMINE BY RAT-LIVER AND KIDNEY CHROMATIN
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DOI：
10.1016/0003-9861(77)90108-4
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发表时间：
1977-01-01
影响因子：
3.9
通讯作者：
WALLE, T
中科院分区：
文献类型：
--
作者：
BLANKENSHIP, J;WALLE, T
在体外酶乙酰化的多胺，亚精胺和精胺，描述。该反应由来自大鼠肝脏和肾脏的染色质制剂催化，并且依赖于乙酰辅酶A。亚精胺、精胺和腐胺均转化为相应的单乙酰衍生物; s0.5值为0.5 .+-。0.1，1.0 .+-。0.1和2.6 .+-。0.7 mM（平均值±. SD）分别获得亚精胺、精胺和腐胺。这些值为s0.5是相似的多胺的浓度报告的组织，并建议在体内多胺乙酰化的发生。还提供了100，000 g大鼠肝脏上清液部分代谢乙酰化多胺的证据。多胺乙酰化的生理功能是未知的，但多胺与核酸的协会的影响的可能性进行了讨论。
The in vitro enzymatic acetylation of the polyamines, spermidine and spermine, is described. The reaction is catalyzed by chromatin preparations from rat liver and kidney and is dependent on acetyl-CoA. Spermidine, spermine and putrescine are each converted to the corresponding monoacetyl derivatives; s0.5 values of 0.5 .+-. 0.1, 1.0 .+-. 0.1 and 2.6 .+-. 0.7 mM (mean .+-. SD) were obtained for spermidine, spermine and putrescine, respectively. These values for s0.5 are similar to the concentrations of polyamines reported for tissues, and suggest the occurrence of polyamine acetylation in vivo. Evidence is also presented for the metabolism of acetylated polyamines by the 100,000 g supernatant fraction of rat liver. The physiological function of polyamine acetylation is unknown, but the possibility of an effect on the association of polyamines with nucleic acids is discussed.