Productive and non-productive phases during long-term persistence of influenza C virus.
Productive and non-productive phases during long-term persistence of influenza C virus.
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丙型流感病毒长期持续存在期间的生产期和非生产期。
DOI：
10.1099/0022-1317-74-9-2019
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
H. Meier
中科院分区：
文献类型：
--
作者：
M. Marschall;C. Böswald;A. Schuler;E. Youzbashi;H. Meier
先前已经报道了MDCK细胞中C/安阿伯/1/50流感病毒变体的持续感染。然而，持久性的精确分子机制仍然是未知的。我们表明，释放的活性子代病毒，血凝和乙酰酯酶谱测试，不发生在新鲜接种的MDCK细胞。如免疫沉淀和免疫荧光所示，生产性病毒复制与结构蛋白的大量生产同时发生。HEF结构蛋白编码片段4的PCR显示，正义RNA仅在病毒增殖期间存在，而反义RNA似乎是不断检测到的。在这项研究中，我们给出了初步的证据表明，丙型流感病毒可以坚持其基因组负链RNA的形式，和正链转录，蛋白质合成和病毒复制仍然限于生产阶段。
Persistent infection with a variant of influenza C/Ann Arbor/1/50 virus in MDCK cells has been previously reported. However, the precise molecular mechanism of persistence is still unknown. We show that the release of active progeny virus, as tested for by haemagglutination and acetylesterase profiles, does not take place in freshly seeded MDCK cells. Productive virus replication occurs simultaneously with massive production of structural proteins as shown by immunoprecipitation and immunofluorescence. PCR for the HEF structural protein-encoding segment 4 revealed that positive-sense RNA is present only during virus multiplication whereas negative-sense RNA appears to be constantly detectable. In this study we give initial evidence that influenza C virus can persist in the form of its genomic minus strand RNA, and plus strand transcription, protein synthesis and virus replication remain restricted to productive phases.
甲型流感病毒持续感染：病毒突变体的进化。
影响因子：
3.7
作者：
Frielle,DW;Huang,DD;Youngner,JS
通讯作者：
Youngner,JS