INTRODUCTION OF A REPORTER GROUP AT ACTIVE SITE OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
INTRODUCTION OF A REPORTER GROUP AT ACTIVE SITE OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
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DOI：
10.1016/0006-291x(66)90559-6
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发表时间：
1966-01-01
影响因子：
3.1
通讯作者：
KOSHLAND, DE
中科院分区：
文献类型：
--
作者：
KIRTLEY, ME;KOSHLAND, DE
结果与讨论 3-磷酸甘油醛脱氢酶被选用于这些研究，因为它的结构已被广泛研究（Velick 和 Furfine，1963；Perham 和 Harris，1963，1965），它由亚基组成，并且动力学研究表明了构象效应（Furfine 和 Velick，1965）。 Theorell (1965) 的精美研究进一步表明构象变化在脱氢酶中可能很重要，他使用了多种技术来证明乙醇脱氢酶中的构象效应。
RESULTS AND DISCUSSION Glyceraldehyde-3-phosphate dehydrogenase was chosen for these studies because its structure has been extensively studied(Velick and Furfine, 1963; Perham and Harris, 1963, 1965), it is composed of subunits, and conformational effects have been suggested from kinetic studies(Furfine and Velick, 1965). A further indication that conformation changes may be important in the dehydrogenases is given by the elegant studies of Theorell(1965) who used a wide variety of techniques to demonstrate conformational effects in alcohol dehydrogenase.