HISTORY OF ELECTROPHORETIC METHODS
HISTORY OF ELECTROPHORETIC METHODS
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DOI：
10.1016/s0021-9673(01)84276-x
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发表时间：
1989-10-20
期刊：
影响因子：
--
通讯作者：
VESTERBERG, O
中科院分区：
文献类型：
--
作者：
VESTERBERG, O
电泳是带电粒子或离子在外加电场作用下在溶液中的迁移。特别是自1950年以来，由于在纸上以及后来在聚丙烯酰胺或琼脂糖凝胶中引入了区带电泳，用于分析和制备目的的分离非常相似的物质（包括不同的蛋白质）的能力有所提高。1960年后，圆盘电泳、置换电泳（等速电泳）和等电聚焦提供了更高的分辨率。电泳方法如今促进了生物化学和分子生物学的进步，并且在科学中以及在遗传学、基因技术、核酸和蛋白质的测序、包括癌症在内的疾病和故障的研究、以及在物种和个体的鉴定（例如，在法医学上。
Electrophoresis is the migration of electrically charged particles or ions in solutions due to an applied electric field. The ability to separate very similar substances including different proteins for analytical and preparative purposes has increased, especially since 1950, owing to the introduction of zone electrophoresis in paper and later in gels of polyacrylamide or agarose. After 1960, disc and displacement electrophoresis (isotachophoresis) and isoelectric focusing offered much increased resolution. Electrophoretic methods nowadays promote advances in biochemistry and molecular biology and will continue to be very important in science and for numerous applications in genetics, gene technology, sequencing of nucleic acids and proteins, studies of diseases and malfunctions including cancer, and in the identification of species and individuals,e.g., in forensic medicine.