Fruit-specific lectins from banana and plantain
Fruit-specific lectins from banana and plantain
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DOI：
10.1007/s004250000307
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发表时间：
2000-09-01
期刊：
影响因子：
4.3
通讯作者：
Van Damme, EJM
中科院分区：
文献类型：
--
作者：
Peumans, WJ;Zhang, WL;Van Damme, EJM
成熟香蕉（Musa acuminata L.）和大蕉（Musa spp.）被鉴定为凝集素。香蕉和大蕉凝集素（分别称为BanLec和PlanLec）纯化在合理的数量使用一种新的分离程序，防止吸附的凝集素不溶性内源性多糖。BanLec和PlanLec都是由两个相同的15 kDa亚基组成的二聚体蛋白。它们容易凝集兔红细胞并对甘露糖表现出特异性。分子克隆显示，BanLec具有序列相似性，以前描述的家庭的jacalin相关的凝集素凝集素，并根据分子模拟研究具有相同的整体折叠和三维结构。BanLec和PlanLec的鉴定证明了在单子叶植物中存在与jacalin相关的凝集素，这表明这些凝集素在高等植物中的分布比实际上所认为的更广泛。香蕉和大蕉凝集素也是第一个记录的例子jacalin相关的凝集素，这是大量存在于成熟果实的果肉，但显然是缺乏其他组织。然而，在用茉莉酸甲酯处理完整植物后，BanLec在叶中也明显被诱导。香蕉凝集素是一种强大的鼠T细胞有丝分裂原。香蕉凝集素的促有丝分裂的相关性进行了讨论，在两个方面的凝集素的生理作用和对食品安全的影响。
One of the predominant proteins in the pulp of ripe bananas (Musa acuminata L.) and plantains (Musa spp.) has been identified as a lectin. The banana and plantain agglutinins (called BanLec and PlanLec, respectively) were purified in reasonable quantities using a novel isolation procedure, which prevented adsorption of the lectins onto insoluble endogenous polysaccharides. Both BanLec and PlanLec are dimeric proteins composed of two identical subunits of 15 kDa. They readily agglutinate rabbit erythrocytes and exhibit specificity towards mannose. Molecular cloning revealed that BanLec has sequence similarity to previously described lectins of the family of jacalin-related lectins, and according to molecular modelling studies has the same overall fold and three-dimensional structure. The identification of BanLec and PlanLec demonstrates the occurrence of jacalin-related lectins in monocot species, suggesting that these lectins are more wide-spread among higher plants than is actually believed. The banana and plantain lectins are also the first documented examples of jacalin-related lectins, which are abundantly present in the pulp of mature fruits but are apparently absent from other tissues. However, after treatment of intact plants with methyl jasmonate, BanLec is also clearly induced in leaves. The banana lectin is a powerful murine T-cell mitogen. The relevance of the mitogenicity of the banana lectin is discussed in terms of both the physiological role of the lectin and the impact on food safety.