Brucellosis of the common vole (Microtus arvalis)
Brucellosis of the common vole (Microtus arvalis)
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DOI：
10.1089/vbz.2007.0143
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发表时间：
2007-12-01
影响因子：
2.1
通讯作者：
Nesvadbova, J.
中科院分区：
文献类型：
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作者：
Hubalek, Z.;Scholz, H. C.;Nesvadbova, J.
1999-2003年间，在南摩拉维亚（捷克共和国）的一个普通田鼠野生种群中发生了一种全身性疾病。急性感染的特征是四肢水肿，偶尔伴有共液性脓肿、关节炎、淋巴结炎、共液性脓肿引起的皮肤穿孔、睾丸炎和腹膜肉芽肿。从临床样本中分离出革兰氏阴性小球芽杆菌，经API 20NE和粘菌素敏感性分析鉴定为Ochrobactrum中间体。但随后对两株分离菌（CCM 4915 = CAPM 6434; CCM 4916 = CAPM 6435）进行rrs （16S rRNA）和recA（重组酶A）基因测序分析，鉴定为布鲁氏菌属，序列与其他布鲁氏菌属同源性100%，omp2a/b基因分析证实为布鲁氏菌。在AMOS聚合酶链反应（PCR）中，产生了一个在其他布鲁氏菌中未见的2000 bp的片段。用这些分离物对近交ICR小鼠进行实验感染，死亡率为50%。根据分子研究结果和实验感染小鼠的死亡率，我们认为这次动物流行病是由布鲁氏杆菌引起的，而不是由中间赭杆菌引起的。该研究证明了商用生化检测系统在准确区分褐杆菌和布鲁氏菌方面的局限性。
A systemic disease occurred in a wild population of the common vole Microtus arvalis in South Moravia (Czech Republic) during the years 1999-2003. Acute infections were characterized by edema of extremities, occasionally with colliquating abscesses, arthritis, lymphadenitis, perforations of the skin resulting from colliquated abscesses, orchitis, and peritoneal granulomas. From the clinical samples, small Gram-negative coccobacilli were isolated and identified as Ochrobactrum intermedium by API 20NE and colistin sensitivity profiles. However, subsequent rrs (16S rRNA) and recA (recombinase A) gene sequencing analysis of two isolates (CCM 4915 = CAPM 6434; CCM 4916 = CAPM 6435) identified them as Brucella sp. with sequence identities of 100% to other Brucella spp. Analysis of the omp2a/b genes confirmed the two isolates as Brucella. In AMOS polymerase chain reaction (PCR), a 2000-bp fragment was generated that was not seen in other brucellae. Experimental infection of outbred ICR mice with these isolates resulted in a mortality rate of 50%. Based on the results of the molecular investigations and the mortality observed in experimentally infected mice we conclude that the epizootic was caused by Brucella sp. and not by Ochrobactrum intermedium. The study demonstrates the limitations of commercial biochemical test systems in accurately differentiating among Ochrobactrum and Brucella.