SHARP‐2/Stra13/DEC1 as a potential repressor of phosphoenolpyruvate carboxykinase gene expression
SHARP‐2/Stra13/DEC1 as a potential repressor of phosphoenolpyruvate carboxykinase gene expression
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DOI：
10.1016/j.febslet.2005.01.060
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发表时间：
2005-02
期刊：
影响因子：
3.5
通讯作者：
Kazuya Yamada;Hiroko Ogata-Kawata;K. Matsuura;K. Miyamoto
中科院分区：
文献类型：
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作者：
Kazuya Yamada;Hiroko Ogata-Kawata;K. Matsuura;K. Miyamoto
研究了分裂毛相关蛋白2增强子(SHARP-2)转录抑制子对大鼠磷酸烯醇丙酮酸羧激酶(PEPCK)基因表达的影响。当用表没食子儿茶素没食子酸酯（一种绿茶成分）处理 H4IIE 细胞时，观察到 SHARP-2 mRNA 水平增加和 PEPCK mRNA 水平降低。 H4IIE细胞和原代培养的大鼠肝细胞中腺病毒介导的SHARP-2过度表达导致PEPCK mRNA水平下降。最后，当SHARP-2表达质粒与各种报告质粒瞬时转染到MH1C1细胞中时，PEPCK报告质粒的启动子活性特异性降低。基于这些发现，我们得出结论，SHARP-2 是 PEPCK 基因表达的潜在抑制因子。
The influence of the enhancer of split- and hairy-related protein-2 (SHARP-2) transcriptional repressor on the expression of rat phosphoenolpyruvate carboxykinase (PEPCK) gene was examined. When H4IIE cells were treated with epigallocatechin gallate, a green tea constituent, an increase in SHARP-2 mRNA levels and a decrease in PEPCK mRNA levels were observed. The adenovirus-mediated overexpression of SHARP-2 in H4IIE cells and primary cultured rat hepatocytes led to a decrease in the levels of PEPCK mRNA. Finally, when a SHARP-2 expression plasmid was transiently transfected with various reporter plasmids into MH1C1cells, the promoter activity of a PEPCK reporter plasmid was specifically decreased. Based on these findings, we conclude that SHARP-2 is a potential repressor of PEPCK gene expression.