Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: Correlation with clinical severity in children in south India
Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: Correlation with clinical severity in children in south India
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DOI：
10.1002/jmv.20053
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发表时间：
2004-05-01
影响因子：
12.7
通讯作者：
Gray, J
中科院分区：
文献类型：
--
作者：
Kang, G;Iturriza-Gomara, M;Gray, J
轮状病毒的流行病学和发病机制尚未完全了解，虽然聚合酶链反应（PCR）技术的最新发展，现在可以量化病毒载量在感染发作，并调查其相关性的疾病的临床特征。我们研究了轮状病毒阳性粪便样本收集自10名儿童没有症状的胃肠炎和81名儿童急性胃肠炎，并在其中的疾病的临床严重程度进行了记录。采用半定量实时逆转录（RT）-PCR来估计轮状病毒载量，并评估其与腹泻严重程度Vesikari评分的相关性。相关分析显示，与血浆中的总蛋白呈显著负相关（r=-0.80，P
The epidemiology and pathogenesis of rota-viruses are not completely understood, although recent developments in polymerase chain reaction (PCR) techniques now make it possible to quantify the viral load during an infective episode and investigate its relevance to clinical features of the disease. We studied rotavirus-positive stool samples collected from 10 children without symptoms of gastroenteritis and from 81 children with acute gastroenteritis and in whom the clinical severity of disease was recorded. A semi-quantitative real-time reverse-transcription (RT)-PCR was used to estimate the rotavirus load and to assess its correlation with the Vesikari score for severity of diarrhoea. There was a significant negative correlation (r=-0.80, P