Cell-surface vimentin-positive macrophage-like circulating tumor cells as a novel biomarker of metastatic gastrointestinal stromal tumors
Cell-surface vimentin-positive macrophage-like circulating tumor cells as a novel biomarker of metastatic gastrointestinal stromal tumors
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细胞表面波形蛋白阳性巨噬细胞样循环肿瘤细胞作为转移性胃肠道间质瘤的新型生物标志物
DOI：
10.1080/2162402x.2017.1420450
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发表时间：
2018-01-01
期刊：
影响因子：
7.2
通讯作者：
Li, Shulin
中科院分区：
文献类型：
--
作者：
Li, Heming;Meng, Qing H.;Li, Shulin
许多文献对循环肿瘤细胞(CTCs)的临床应用进行了研究，但表达非常典型的免疫细胞标志物的CTCs尚未见报道。在这里，我们报告了一类新的CTCs-CSV阳性巨噬细胞样CTCs(ML-CTCs)。这一命名是基于这样一个事实，即可以使用巨噬细胞标志物CD68或我们专有的肿瘤特异性细胞表面波形蛋白(CSV)抗体84-1从胃肠道间质瘤(GIST)患者的血液样本中捕获此类CTCs；同样，捕获的ML-CTCs可以与典型的巨噬细胞标志物(CD14、CD68)和肿瘤细胞标志物(DOG-1、C-KIT)共染色，但不能与CD45共染色。转移性GIST患者的ML-CTCs数量明显多于局限型GIST患者和无癌献血者(P
The clinical utility of circulating tumor cells (CTCs) has been investigated in numerous publications, but CTCs that express very typical immune cell markers have not been reported. Here we report a novel class of CTCs-CSV-positive macrophage-like CTCs (ML-CTCs). This nomenclature was based on the fact that this class of CTCs can be captured from blood samples of gastrointestinal stromal tumors (GISTs) patients using either the macrophage marker CD68 or our proprietary tumor-specific cell-surface vimentin (CSV) antibody 84-1; likewise, the captured ML-CTCs can be co-stained with both typical macrophage markers (CD14, CD68) and tumor cell markers (DOG-1, C-kit) but not CD45. Patients with metastatic GIST had significantly greater numbers of ML-CTCs than patients with localized GIST or cancer-free blood donors (P