AUTORADIOGRAPHY OF CHROMOSOMAL DNA FIBERS FROM CHINESE HAMSTER CELLS
AUTORADIOGRAPHY OF CHROMOSOMAL DNA FIBERS FROM CHINESE HAMSTER CELLS
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DOI：
10.1073/pnas.55.3.599
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发表时间：
1966-01-01
影响因子：
11.1
通讯作者：
RIGGS, AD
中科院分区：
文献类型：
--
作者：
HUBERMAN, JA;RIGGS, AD
当凯恩斯技术应用于中国仓鼠细胞时，发现了线性DNA放射自显影。这些放射自显影中至少有6%的长度超过0.8毫米-大致相当于大肠杆菌染色体的大小，比之前报道的来自高等生物的DNA纤维要长得多。一些罕见的放射自显影长达1.6-1.8毫米。讨论了这些结果在染色体结构模型方面的意义。
Linear DNA autoradiograms are found when the Cairns technique is applied to Chinese hamster cells. At least 6% of these autoradiograms are more than 0.8 mm long-roughly the size of the Escherichia coli chromosome and considerably longer than previously reported DNA fibers from higher organisms. Some rare autoradiograms are as long as 1.6-1.8 mm. The implications of these results in terms of models of chromosome structure are disc ussed.