Human rhinoviruses in otitis media with effusion
Human rhinoviruses in otitis media with effusion
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DOI：
10.1111/j.1399-3038.2006.00448.x
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发表时间：
2006-11-01
影响因子：
4.4
通讯作者：
Kafetzis, D. A.
中科院分区：
文献类型：
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作者：
Chantzi, F. M.;Papadopoulos, N. G.;Kafetzis, D. A.
频繁的病毒性上呼吸道感染（URTI）被认为是渗出性中耳炎（OME）的危险因素。特应性也与 OME 和病毒感染有关。本研究的目的是评估 2-7 岁患有 OME 的儿童中耳积液 (MEE) 中病毒的存在情况，并确定 MEE 中病毒检测的危险因素。对 37 名 OME 儿童在鼓膜切开术时收集的 MEE 样本进行了评估。还进行了体格检查、皮肤点刺测试以及关于 OME 和过敏的标准化问卷。通过逆转录PCR (RT-PCR) 检测病毒RNA。 15 个样本（40.5%）鼻病毒（RV）呈阳性。检测到一种肠道病毒，未检测到其他呼吸道病毒。在急性、亚急性和慢性病例中，分别有五分之二 (40%)、3/7 (43%) 和 10/25 (40%) 的 RV 呈阳性。 OM 频繁发作、OM 发病较早的儿童（
Frequent viral upper respiratory tract infections (URTI) are considered to be risk factors for otitis media with effusion (OME). Atopy has also been associated with both OME and viral infections. The aim of this study was to evaluate the presence of viruses in middle ear effusions (MEE) in children 2-7 yr old with OME, and to determine risk factors for virus detection in the MEE. MEE samples, collected at the time of myringotomy from 37 children with OME were assessed. Physical examination, skin prick tests and a standardized questionnaire on OME and allergy were also performed. Viral RNA was detected by the use of reverse transcription PCR (RT-PCR). Fifteen samples (40.5%) were positive for rhinovirus (RV). One enterovirus and no other respiratory viruses were detected. Two out of five (40%), 3/7 (43%) and 10/25 (40%) were positive for RV in acute, subacute and chronic cases, respectively. Children with frequent episodes of OM, with early onset of OM (