Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.
Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.
复制标题
脑啡肽抑制培养物中感觉神经元释放 P 物质并减少动作电位持续时间。
DOI：
--
复制
发表时间：
1979
影响因子：
11.1
通讯作者：
G. Fischbach
中科院分区：
文献类型：
--
作者：
A. Mudge;S. Leeman;G. Fischbach
在缺乏非神经元细胞类型的分散细胞培养物中生长的感觉神经元含有免疫反应物质 P，其化学性质与合成物质 P 相似。当在高 K+ 介质 (30-120 mM) 中去极化时，神经元通过 Ca2+ 依赖性机制释放这种肽。脑啡肽类似物 [D-Ala2] 脑啡肽酰胺在 10 微米时可抑制 K+ 诱发的 P 物质释放。在相同或更低的浓度下，[D-Ala2] 脑啡肽酰胺和脑啡肽可缩短背根神经节细胞体中诱发和记录的 Ca2+ 动作电位的持续时间，而不影响静息膜电位或静息膜电导。这种电压敏感通道的调节可能是抑制 P 物质释放的原因。
Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+ media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 micron inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+ action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. This modulation of voltage-sensitive channels may account for the inhibition of substance P release.