A Ganoderma lucidum polysaccharide F31 alleviates hyperglycemia through kidney protection and adipocyte apoptosis
A Ganoderma lucidum polysaccharide F31 alleviates hyperglycemia through kidney protection and adipocyte apoptosis
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灵芝多糖F31通过肾脏保护和脂肪细胞凋亡缓解高血糖
DOI：
10.1016/j.ijbiomac.2022.11.231
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发表时间：
2022-12-29
影响因子：
8.2
通讯作者：
Liu，Rongjie
中科院分区：
文献类型：
--
作者：
Jiao，Jiaqi;Yong，Tianqiao;Liu，Rongjie
在本文中，我们报道了灵芝多糖 F31 具有优异的降血糖作用，功效在 45% 至 54% 之间，接近利拉鲁肽 (52%)。值得注意的是，F31 减少了体重增加和食物摄入量。 F31降低了4种关键化合物，包括腺苷、腺苷、半乳糖醇和甘油磷酸胆碱，升高了8种关键化合物，包括精氨酸、脯氨酸、花生四烯酸、肌酸、天冬氨酸、亮氨酸、苯丙氨酸和鸟氨酸，从而保护肾功能。此外，F31 通过增加 Caspase-3、Caspase-6 和 Bax 并减少 Bcl-2 来促进附睾脂肪中的细胞凋亡。在 3 T3-L1 前脂肪细胞中，F31 通过降低线粒体膜电位诱导早期细胞凋亡。最后，进行分子对接以揭示肾脏和附睾脂肪之间通过甘油磷酸胆碱-Bax 轴的可能的串扰。总体而言，F31 通过肾脏保护和脂肪细胞凋亡减轻 db/db 小鼠的高血糖。这项工作可能为多糖的降血糖活性提供新的见解。
In this paper, we reported an excellent hypoglycemic effect of a Ganodermalucidiumpolysaccharide F31 with efficacies between 45 and 54 %, approaching to that of liraglutide (52 %). Significantly, F31 reduced the body weight gains and food intakes. F31 decreased 4 key compounds, consisting of adenosine, adenosine, galactitol and glycerophosphocholine and elevated 8 key compounds, including arginine, proline, arachidonic acid, creatine, aspartic acid, leucine, phenylalanine and ornithine, which protected kidney function. Also, apoptosis was promoted by F31 in epididymal fat through increasing Caspase-3, Caspase-6 and Bax and decreasing Bcl-2. On 3 T3-L1 preadipocyte cells, F31 induced early apoptosis through reducing mitochondrial membrane potential. Finally, a molecular docking was performed to reveal a plausible cross-talk between kidney and epididymal fat through glycerophosphorylcholine-Bax axis. Overall, F31 alleviated hyperglycemia through kidney protection and adipocyte apoptosis in db/db mice. This work may provide novel insights into the hypoglycemic activity of polysaccharides.