Synapsis and meiotic recombination analyses:: MLH1 focus in the XY pair as an indicator
Synapsis and meiotic recombination analyses:: MLH1 focus in the XY pair as an indicator
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DOI：
10.1093/humrep/dei023
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发表时间：
2005-08-01
影响因子：
6.1
通讯作者：
Benet, J
中科院分区：
文献类型：
--
作者：
Codina-Pascual, M;Oliver-Bonet, M;Benet, J
背景：减数分裂前期I的异常与部分或全部减数分裂停滞有关。这些异常包括异常的突触过程，导致减数分裂重组的障碍。方法：在本研究中，我们分析了初级精母细胞从12个不育男性（4个非梗阻性无精子症，6个寡astenoteratozomia，一个astenoteratozomia和一个normozomic）和5个控制生育捐助者使用联会复合体，减数分裂重组和着丝粒蛋白的免疫细胞学技术。研究结果：报告了每个不育患者和对照男性的每个细胞的MLH1病灶的平均数量、XY对中呈现MLH1病灶的细胞的频率和受异常突触模式（间隙和分裂）影响的细胞的百分比。一个正相关的频率之间的细胞显示在XY对重组焦点和每cell的常染色体重组focuses的数量是found.CONCLUSIONS：减少重组的XY对和增加数量的细胞受影响的差距可能解释一些特发性男性不育症的情况。结果表明，XY对中的重组可能是一般重组频率和成功减数分裂过程的指标。
BACKGROUND: Anomalies in meiotic prophase I have been related to partial or total meiotic arrest. These anomalies include an abnormal synaptic process, resulting in disorders in meiotic recombination. METHODS: In the present study, we analyse primary spermatocytes from 12 infertile men (four with non-obstructive azoospermia, six with oligoastenoteratozoospermia, one with astenoteratozoospermia and one normozoospermic) and five control fertile donors using immunocytological techniques for synaptonemal complex, meiotic recombination and centromeric proteins. RESULTS: Mean numbers of MLH1 foci per cell, frequencies of cells presenting an MLH1 focus in the XY pair and percentages of cells affected by abnormal synaptic patterns (gaps and splits) are reported for each of the infertile patients and control men. A positive correlation between the frequency of cells showing a recombination focus in the XY pair and the number of autosomal recombination foci per cell is found. CONCLUSIONS: Reduced recombination in the XY pair and an increased number of cells affected by gaps may explain some idiopathic male infertility cases. The results suggest that recombination in the XY pair could be an indicator for general recombination frequency and for a successful meiotic process.