Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor.
Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor.
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DOI：
10.1083/jcb.120.2.325
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发表时间：
1993-01
期刊：
影响因子：
--
通讯作者：
Hong W
中科院分区：
文献类型：
--
作者：
Tang BL;Wong SH;Qi XL;Low SH;Hong W
我们分离了酵母 ERD2 基因的哺乳动物（牛）同源物的 cDNA 克隆 (mERD2)，该基因编码酵母 HDEL 受体。推导的氨基酸序列与其酵母对应物具有广泛的同源性，并且与先前描述的人类序列几乎相同。该序列预测出一种具有多个跨膜结构域的高度疏水性蛋白质，正如体外翻译产物分析所证实的那样。 mERD2 (p23) 编码的蛋白质广泛存在，存在于所有检查的细胞类型中。 p23 定位于高尔基体的顺侧和介导 ER 至高尔基体转运的斑点状中间室。大多数细胞内染色可通过低温（15°C）或布雷菲德菌素 A 积累在中间室中。在从这些处理恢复期间，p23 的点状中间室染色转移到高尔基体的核周染色，并观察到 p23 标记的管状结构。这些管状结构可用于介导中间室和高尔基体之间的运输。
We have isolated a cDNA clone (mERD2) for the mammalian (bovine) homologue of the yeast ERD2 gene, which codes for the yeast HDEL receptor. The deduced amino acid sequence bears extensive homology to its yeast counterpart and is almost identical to a previously described human sequence. The sequence predicts a very hydrophobic protein with multiple membrane spanning domains, as confirmed by analysis of the in vitro translation product. The protein encoded by mERD2 (p23) has widespread occurrence, being present in all the cell types examined. p23 was localized to the cis-side of the Golgi apparatus and to a spotty intermediate compartment which mediates ER to Golgi transport. A majority of the intracellular staining could be accumulated in the intermediate compartment by a low temperature (15 degrees C) or brefeldin A. During recovery from these treatments, the spotty intermediate compartment staining of p23 was shifted to the perinuclear staining of the Golgi apparatus and tubular structures marked by p23 were observed. These tubular structures may serve to mediate transport between the intermediate compartment and the Golgi apparatus.