Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
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DOI：
10.1007/978-1-59745-019-5_11
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Smalley, Matthew J.
中科院分区：
文献类型：
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作者：
Smalley, Matthew J.
对乳腺癌和乳腺癌细胞生物学的有限了解阻碍了我们开发新的治疗方法的能力。小鼠乳腺发育和肿瘤发生的模型是发展对正常组织和病变组织生物学的新见解的关键。最近的进展使小鼠乳腺上皮细胞亚群的分离、分子特征和功能分析从正常腺体中分离出来，包括为干细胞行为而丰富的亚群。将这些技术应用于正常乳腺和肿瘤模型，将有助于更好地了解乳腺中不同上皮细胞类型的性质、乳腺肿瘤的起源以及肿瘤干细胞的作用。
Limited understanding of the cell biology of the breast and breast cancer hampers our ability to develop new therapeutic approaches. Mouse models of mammary gland development and tumourigenesis arc key to developing new insights into the biology of both the normal and diseased tissues. Recent advances have enabled the isolation, molecular characterisation and functional analysis of mouse mammary epithelial cell subpopulations from the normal gland, including subpopulations enriched for stem cell behaviour. Application of these techniques both to the normal mammary gland and to tumour models will promote a better understanding of the nature of the different epithelial cell types in the mammary gland, the origins of mammary tumours and the role of tumour stem cells.