Photoperiod-insensitive Japanese soybean landraces differ at two maturity loci
Photoperiod-insensitive Japanese soybean landraces differ at two maturity loci
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DOI：
10.2135/cropsci2003.1300
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发表时间：
2003-07-01
期刊：
影响因子：
2.3
通讯作者：
Shimamoto, Y
中科院分区：
文献类型：
--
作者：
Abe, J;Xu, DH;Shimamoto, Y
开花对长日照不敏感是大豆[Glycine mar（L.）梅里尔]到高纬度环境。本研究的目的是确定和定位两个地方品种，“Miharudaizu”和“Sakamotowase”，这属于不同的品种群的白炽灯长日照（ILD）不敏感的成熟基因。利用重复杂合子选择法，从两个地方品种杂交的F-2群体中选育出两个F-9重组自交系（RIL）家系。同工酶和SSR标记的连锁分析表明，三春代猪ILD不敏感性的成熟基因是位于MLG Ⅰ上E4位点的隐性等位基因。E4位点与4个标记的MLG Ⅰ顺序为Satt 239-Satt 496-E4-Enp-Satt 354。发现Sakamotowase的ILD不敏感性成熟基因与MLG C2上的4个紧密连锁的SSR共分离，并确定为El位点的隐性等位基因。在E3位点的基因型Miharudaizu和Sakamotowase估计为e3 e3的基础上，晚花近等基因系荧光长日照（FLD）的反应。本研究结果表明，Miharudaizu的基因型为E1 E1 e3 e3 e4 e4，Sakamotowase的基因型为e1 e1 e3 e3 E4 E4。由于e1等位基因不能在E4等位基因存在的情况下诱导ILD开花，一个未知的基因可能会调节Sakamotowase的ILD不敏感性，可能与e1等位基因结合。
Insensitivity of flowering to long daylengths is an important character in the adaptation of soybean [Glycine mar (L.) Merrill] to higher latitudinal environments. The objective of this study was to identify and map the maturity genes for incandescent long daylength (ILD) insensitivity for two landraces, 'Miharudaizu' and 'Sakamotowase', which belong to different cultivar groups. Two F-9 recombinant inbred line (RIL) families were developed by means of a repetitive heterozygote selection method from the F-2 population of the cross between the two landraces. Linkage analyses with isozyme and simple sequence repeat (SSR) markers revealed that the maturity gene for ILD insensitivity from Miharudaizu was a recessive allele at the E4 locus on Molecular linkage group (MLG) I. The MLG I order of the E4 locus and four markers was determined as Satt239-Satt496-E4-Enp-Satt354. The maturity gene for ILD insensitivity from Sakamotowase was found to cosegregate with four tightly linked SSRs on MLG C2, and was determined as a recessive allele at the El locus. The genotypes at the E3 locus for both Miharudaizu and Sakamotowase were estimated as e3e3 on the basis of the responses of late-flowering near-isogenic lines to fluorescent long daylength (FLD). The results obtained in this study suggest that Miharudaizu has the genotype of E1E1e3e3e4e4 and Sakamotowase has the genotype of e1e1e3e3E4E4. Because the e1 allele cannot induce flowering in ILD in the presence of the E4 allele, an unknown gene may condition the ILD insensitivity of Sakamotowase, possibly while combined with the e1 allele.