Squalamine ointment for Staphylococcus aureus skin decolonization in a mouse model
Squalamine ointment for Staphylococcus aureus skin decolonization in a mouse model
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DOI：
10.1093/jac/dkr114
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发表时间：
2011-06-01
影响因子：
5.2
通讯作者：
Brunel, Jean Michel
中科院分区：
文献类型：
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作者：
Djouhri-Bouktab, Lamia;Alhanout, Kamel;Brunel, Jean Michel
目的：金黄色葡萄球菌定植的皮肤和鼻孔仍然是一个主要的原因，泌尿系统部位感染，尽管术前和预防程序。迄今为止，许多化合物已被用于S。金黄色葡萄球菌的去殖民化，包括莫匹罗星软膏和防腐剂，具有可变的结果。耐莫匹罗星的S.金黄色葡萄球菌菌株的研究导致了对专门针对金黄色葡萄球菌的新的抗微生物剂的研究。奥里亚非殖民化。在这项工作中，我们评估了角鲨胺和相关的父母衍生的软膏（1%）作为潜在的新化合物的S。方法：我们报道了角鲨胺和相关的母体衍生软膏在新的小鼠皮肤模型中的开发和应用。刮除皮肤后，小鼠被S.金黄色葡萄球菌悬浮液，校准至10（4）-10（6）cfu/mL。在单次涂抹角鲨胺后，通过涂布法监测剩余细菌负荷2天。皮肤的金黄色葡萄球菌定殖在其自然消除之前稳定至少2天。利用该模型，我们发现角鲨胺软膏（1%）可以降低S。金黄色葡萄球菌活细胞数在单次1小时软膏应用中减少了高达4个对数，而莫匹罗星应用在相同时间内仅减少了1.3个对数（P
Objectives: Staphylococcus aureus colonization of the skin and the nostrils remains a major cause of surgical-site infections despite preoperative and preventive procedures. To date, many compounds have been used for S. aureus decolonization, including mupirocin ointments and antiseptics, with variable results. The emergence of mupirocin-resistant S. aureus strains has led to the search for new antimicrobial agents specifically for S. aureus decolonization. In this work we evaluated squalamine and related parent-derived ointments (1%) as potential new compounds for S. aureus decolonization in a new mouse model.Methods: We report the development and application of squalamine and related parent-derived ointments in a new mouse skin model. After skin shaving, mice were colonized with an S. aureus suspension that was calibrated to 10(4)-10(6) cfu/mL. The remaining bacterial load was monitored for 2 days after a single application of squalamine by spreading.Results: We found that S. aureus colonization of the skin was stable for at least 2 days before it was naturally eliminated. Using this model we found that squalamine ointment (1%) could reduce S. aureus viable cells by up to 4 log with a single, 1 h application of ointment, whereas mupirocin application reduced viable cell numbers by only 1.3 log during that same time (P