Evidence for biological effects in the radiosensitization of leukemia cell lines by PEGylated gold nanoparticles
Evidence for biological effects in the radiosensitization of leukemia cell lines by PEGylated gold nanoparticles
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DOI：
10.1007/s11051-020-4765-1
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发表时间：
2020-02-15
影响因子：
2.5
通讯作者：
Mace，Charles R.
中科院分区：
文献类型：
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作者：
Coughlin，Benjamin P.;Lawrence，Paul T.;Mace，Charles R.
金纳米粒子(Au NPs)是一种很有前途的肿瘤放射增敏剂。虽然金纳米粒子在许多上皮性癌症中显示了放射增敏作用，但还没有文献报道将其用于造血细胞，这些细胞占所有癌症病例的10%左右，经常使用放射疗法进行治疗。在这项工作中，我们用流式细胞仪和电感耦合等离子体发射光谱仪测量了聚乙二醇包被(聚乙二醇化)的金纳米颗粒(核心直径5 nm)在HL-60 II和Jurkat D1.1细胞中的摄取。电子细胞计数、代谢活性分析和DNA合成分析表明，细胞系特异性的放射增敏作用与内化纳米颗粒的数量无关。HL-60 II细胞系的高SER值(5Gy时为1.33)表明了一种占主导地位的生物学机制。
Gold nanoparticles (Au NPs) are promising radiosensitizers for cancer therapy. While the radiosensitizing effects of Au NPs have been shown in many epithelial cancers, there are no documented cases of their use in cells of hematopoietic origin, which constitute ~ 10% of all cancer cases and are frequently treated using radiation therapy. In this work, we measure the uptake of polyethylene glycol-coated (PEGylated) Au NPs (5 nm core diameter) in HL-60 II and Jurkat D1.1 cells using flow cytometry and ICP-AES. Electronic cell counting, metabolic activity assays, and DNA synthesis assays reveal cell line–specific radiosensitization that is independent of the number of internalized nanoparticles. The high SER value for the HL-60 II cell line (1.33 at 5 Gy) points to a dominant biological mechanism.